SNX8 Antibody (OTI1E5) - Azide and BSA Free

Western Blot: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNX8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNX8.

Immunocytochemistry/Immunofluorescence: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNX8.

Immunohistochemistry: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - Staining of paraffin-embedded Human lymph node tissue using anti-SNX8 mouse monoclonal antibody.

Flow Cytometry: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SNX8 antibody, and then analyzed by flow cytometry.

Western Blot: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SNX8 monoclonal antibody.

Immunohistochemistry: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SNX8 mouse monoclonal antibody.

Immunohistochemistry: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNX8 mouse monoclonal antibody.

Immunohistochemistry: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SNX8 mouse monoclonal antibody.

Immunohistochemistry: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - Staining of paraffin-embedded Human Kidney tissue using anti-SNX8 mouse monoclonal antibody.

Immunohistochemistry: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - Staining of paraffin-embedded Human liver tissue using anti-SNX8 mouse monoclonal antibody.

Immunohistochemistry: SNX8 Antibody (OTI1E5) - Azide and BSA Free [NBP2-74266] - Staining of paraffin-embedded Human thyroid tissue using anti-SNX8 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SNX8 Antibody (OTI1E5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SNX8 Antibody (OTI1E5) - Azide and BSA Free (NBP2-03367) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human SNX8 (NP_037453) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SNX8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 52.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SNX8 Antibody (OTI1E5) - Azide and BSA Free

• DKFZp761E1721
• Mvp1
• sorting nexin 8
• sorting nexin-8

Background

SNX8 may be involved in several stages of intracellular trafficking

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNX8