SNX1 Antibody

Immunohistochemistry: SNX1 Antibody [NB100-282] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SNX1 used at a dilution of 1:400 (2.5 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.

Immunohistochemistry-Paraffin: SNX1 Antibody [NB100-282] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SNX1 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

Immunohistochemistry: SNX1 Antibody [NB100-282] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SNX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SNX1 Antibody Summary

• Immunogen: A synthetic peptide, which represented a portion of human Sorting Nexin 1 encoded within exon 15 (LocusLink ID 6642).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Western Blot 1:500-1:5000
• Application Notes: Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SNX1 Antibody

• HsT17379
• MGC8664
• SNX1Asorting nexin 1A
• sorting nexin 1
• sorting nexin-1
• Vps5

Background

Sorting nexin 1 is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNX1 Antibody (NB100-282)(2)

Publications using NB100-282

• Nielsen MS, Gustafsen C, Madsen P et al. Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA. Mol Cell Biol 2007-10-01 [PMID: 17646382]
• Kurten RC, Eddington AD, Chowdhury P et al. Self-assembly and binding of a sorting nexin to sorting endosomes. J Cell Sci 2001-05-01 [PMID: 11309204]

Bioinformatics

• Gene Symbol: SNX1