Recombinant Human SNF8 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human SNF8 Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 258 of Human SNF8

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMHRRGV GAGAIAKKKL AEAKYKERGT VLAEDQLAQM SKQLDMFKTN LEEFASKHKQ EIRKNPEFRV QFQDMCATIG VDPLASGKGF WSEMLGVGDF YYELGVQIIE VCLALKHRNG GLITLEELHQ QVLKGRGKFA QDVSQDDLIR AIKKLKALGT GFGIIPVGGT YLIQSVPAEL NMDHTVVLQL AEKNGYVTVS EIKASLKWET ERARQVLEHL LKEGLAWLDL QAPGEAHYWL PALFTDLYSQ EITAEEAREA LP
Protein/Peptide Type
Recombinant Protein
Gene
SNF8
Purity
>90%, by SDS-PAGE

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer, pH8.0, 50% glycerol, 2mM DTT, 200mM NaCl
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
This product is useful for SDS-PAGE. The purity of this protein is > 90% by SDS - PAGE.
Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Alternate Names for Recombinant Human SNF8 Protein

  • EAP30 subunit of ELL complex
  • EAP30hVps22
  • ELL-associated protein of 30 kDa
  • ESCRT-II complex subunit VPS22
  • SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
  • vacuolar-sorting protein SNF8
  • VPS22Dot3

Background

SNF 8, also known as EAP30 (ELL-associated protein of 30 kDa), is localizes to both the nucleus and the cytoplasm and is a member of the SNF8 family of vacuolar sorting proteins. This protein is subunit of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Recombinant human SNF8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SNF8

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