SNF8 Antibody

Product Discontinued

SNF8 Antibody Summary

• Immunogen: A synthetic peptide from the internal part of human Vacuolar-sorting protein SNF8 (EAP30, SNF8, ELL-associated protein of 30 kDa) conjugated to an immunogenic carrier protein was used as the immunogen.
• Localization: subcellular location: cytoplasm. nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNF8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 10-50 ug/ml
  • Immunohistochemistry-Paraffin 10-50 ug/ml
  • Western Blot 10-50 ug/ml
• Control:

  SNF8 Overexpression Lysate

Reactivity Notes

The antigen is homologous in Rat, Mouse and Xenopus.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: IgG purified
• Reconstitution Instructions: Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for SNF8 Antibody

• EAP30 subunit of ELL complex
• EAP30hVps22
• ELL-associated protein of 30 kDa
• ESCRT-II complex subunit VPS22
• SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
• vacuolar-sorting protein SNF8
• VPS22Dot3

Background

Component of the escrt-ii complex, which is required for multivesicular bodies (mvbs) formation and sorting of endosomal cargo proteins into mvbs. the mvb pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. the escrt-ii complex is probably involved in the recruitment of the escrt-iii complex. the escrt-ii complex may also play a role in transcription regulation by participating in derepression of transcription by rna polymerase ii, possibly via its interaction with ell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNF8
• Entrez:
  • 11267(Human)
  • 27681(Mouse)
  • 287645(Rat)
• Uniprot:
  • Q96H20(Human)