SNF5 Antibody (3E10)

Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10. Analysis of SMARCB1 expression in Raw 264.7.

Immunohistochemistry-Paraffin: SNF5 Antibody (3E10) [H00006598-M01] - Analysis of monoclonal antibody to SMARCB1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10 Analysis of SMARCB1 expression in PC-12.

Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10. Analysis of SMARCB1 expression in Hela S3 NE.

Western Blot: SNF5 Antibody (3E10) [H00006598-M01] - SMARCB1 monoclonal antibody (M01), clone 3E10. Analysis of SMARCB1 expression in NIH/3T3.

ELISA: SNF5 Antibody (3E10) [H00006598-M01] - Detection limit for recombinant GST tagged SMARCB1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
• Clone: 3E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SNF5 Antibody (3E10) Summary

• Immunogen: SMARCB1 (NP_003064, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YTTLATSVTLLKASEVEEILDGNDEKYKAVSISTEPPTYLREQKAKRNSQWVPTLPNSSHHLDAVPCSTTINRNRMGRDKKRTFPLCFDDHDPAVIHENA
• Specificity: SMARCB1 - SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMARCB1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SNF5 Antibody (3E10)

• BAF47
• BAF47hSNF5
• BRG1-associated factor 47
• hSNFS
• Ini1
• INI1RTPS1
• Integrase interactor 1 protein
• malignant rhabdoid tumor suppressor
• RDTSNF5 homolog
• Sfh1p
• SNF5
• SNF5L1
• Snr1
• subfamily b, member 1
• sucrose nonfermenting, yeast, homolog-like 1
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily B member 1

Background

The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNF5 Antibody (H00006598-M01)(1)

Publication using H00006598-M01

• Scurr LL, Pupo GM, Becker TM et al. IGFBP7 is not required for B-RAF-induced melanocyte senescence. Cell141(4):717-27. 2010 May 14. [PMID: 20478260]

FAQs for SNF5 Antibody (H00006598-M01). (Showing 1 - 1 of 1 FAQ).

1. Can I use this antibody with paraffin wax embedded tissue of meningioma for Immunohistochemistry?
   • H00006598-M01 has been tested and validated for use in IHC-Paraffin in the following species: human, rat and mouse. The IHC image you can see on the datasheet is of human testis. The ability to detect the protein in the tumor tissues you are studying really depends on the tumor biology. We do guarantee the antibody in human and would highly recommend using a positive control of normal tissue in your staining.

Bioinformatics

• Gene Symbol: SMARCB1
• Entrez:
  • 6598(Human)
• OMIM:
  • 601607(Human)
• Uniprot:
  • NP_003064(Human)