SNAP23 Antibody (JA73-15)

Western Blot: SNAP23 Antibody (JA73-15) [NBP2-67157] - Analysis of SNAP23 on Hela cell using anti-SNAP23 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: SNAP23 Antibody (JA73-15) [NBP2-67157] - Analysis of paraffin-embedded human tonsil tissue using anti-SNAP23 antibody. Counter stained with hematoxylin.

Flow Cytometry: SNAP23 Antibody (JA73-15) [NBP2-67157] - Analysis of Hela cells with SNAP23 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Discontinued

SNAP23 Antibody (JA73-15) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SNAP23 aa 162-211 / 211. (SwissProt: O00161 Human; SwissProt: O09044 Mouse; SwissProt: O70377 Rat)
• Localization: Cell membrane. Cell junction.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SNAP23
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SNAP23 Antibody (JA73-15)

• HsT17016
• SNAP23
• SNAP-23
• SNAP23A
• SNAP23B
• synaptosomal-associated protein 23
• synaptosomal-associated protein, 23kD
• synaptosomal-associated protein, 23kDa
• Vesicle-membrane fusion protein SNAP-23

Background

Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNAP23 Antibody (NBP2-67157)(1)

Publication using NBP2-67157

• Huang M, Bin N, Rai J et al. Neuronal SNAP-23 is critical for synaptic plasticity and spatial memory independently of NMDA receptor regulation iScience 2023-05-01 [PMID: 37168570] (IHC-Fr, Mouse)
  
  Details:
  conjugated with Alexa Fluor 488

Bioinformatics

• Gene Symbol: SNAP23