Smek1 Antibody

Western Blot: Smek1 Antibody [NB100-2879] - Detection of Human and Mouse PPP4R3 Alpha on HeLa whole cell lysate using NB100-2879. PPP4R3 alpha was also immunoprecipitated by rabbit anti-PPP4R3 alpha antibody NB100-2878.

Immunohistochemistry: Smek1 Antibody [NB100-2879] - Sample: FFPE sections of human colon carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- PPP4R3 Alpha used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-he

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Smek1 Antibody Summary

• Description: Novus Biologicals Rabbit Smek1 Antibody (NB100-2879) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 770 and the C-terminus (residue 820) of human Protein Phosphatase 4, Regulatory Subunit 3 Alpha (KIAA2010) using the numbering given in entry NP_115949.3
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP4R3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Control:

  Smek1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Smek1 Antibody

• FLFL1
• FLJ20707
• KIAA2010MSTP033
• PP4R3A
• PPP4R3A
• serine/threonine-protein phosphatase 4 regulatory subunit 3A
• SMEK homolog 1
• SMEK homolog 1, suppressor of mek1 (Dictyostelium)
• smk1
• smk-1

Background

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP4R3A
• Entrez:
  • 55671(Human)
  • 68734(Mouse)
• Uniprot:
  • Q6IN85(Human)