SMC1 Antibody (SN20-27)

Western Blot: SMC1 Antibody (SN20-27) [NBP2-67733] - Analysis of SMC1 on different lysates with Rabbit anti-SMC1 antibody at 1/500 dilution.Lane 1: Hela cell lysateLane 2: 293T cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 143 kDaObserved band size: 143 kDaExposure time: 30 seconds;8% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: SMC1 Antibody (SN20-27) [NBP2-67733] - Staining SMC1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMC1 Antibody (SN20-27) [NBP2-67733] - Analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-SMC1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: SMC1 Antibody (SN20-27) [NBP2-67733] - Analysis of SMC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: Hela cell lysate

Immunocytochemistry/Immunofluorescence: SMC1 Antibody (SN20-27) [NBP2-67733] - Staining SMC1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SMC1 Antibody (SN20-27) [NBP2-67733] - Staining SMC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMC1 Antibody (SN20-27) [NBP2-67733] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-SMC1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMC1 Antibody (SN20-27) [NBP2-67733] - Analysis of paraffin-embedded human tonsil tissue using anti-SMC1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMC1 Antibody (SN20-27) [NBP2-67733] - Analysis of paraffin-embedded mouse colon tissue using anti-SMC1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SMC1 Antibody (SN20-27) [NBP2-67733] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-SMC1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SMC1 Antibody (SN20-27) [NBP2-67733] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-SMC1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SMC1 Antibody (SN20-27) [NBP2-67733] - Analysis of paraffin-embedded rat bladder tissue with Rabbit anti-SMC1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

SMC1 Antibody (SN20-27) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SMC1 aa 1,184-1,233 / 1,233. (SwissProt: Q14683 Human; SwissProt: Q8NDV3 Human; SwissProt: Q920F6 Mouse; SwissProt: Q9CU62 Mouse; SwissProt: Q9Z1M9 Rat)
• Localization: Nucleus, Chromosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMC1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SMC1 Antibody (SN20-27)

• CDLS2
• DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast)
• KIAA0178DKFZp686L19178
• SB1.8
• SB1.8MGC138332
• segregation of mitotic chromosomes 1
• SMC protein 1A
• SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
• SMC1 structural maintenance of chromosomes 1-like 1
• SMC1
• SMC1A
• SMC-1A
• SMC-1-alpha
• SMC1L1
• SMC1SMC1alpha
• SMCB
• structural maintenance of chromosomes 1A
• structural maintenance of chromosomes protein 1A

Background

SMC1 is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of the cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, SMC1 works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMC1 Antibody (NBP2-67733)(1)

Publication using NBP2-67733

• Ochs, F, Karemore, G Et al. Stabilization of chromatin topology safeguards genome integrity. Nature 2019-10-01 [PMID: 31645724] (ICC/IF, Drosophila melanogaster)

FAQs for SMC1 Antibody (NBP2-67733). (Showing 1 - 2 of 2 FAQ).

1. I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?
   • My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.
2. My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.
   • We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.

Bioinformatics

• Gene Symbol: SMC1A