SMARCD1 Overexpression Lysate (Native)

Images

 
Western Blot: SMARCD1 Overexpression Lysate (Adult Normal) [NBL1-16234] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMARCD1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SMARCD1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203474 and based on accession number NM_003076. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 (SMARCD1), transcript variant 1, mRNA.
Gene
SMARCD1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
58.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SMARCD1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SMARCD1 Overexpression Lysate (Native)

  • BAF60Amammalian chromatin remodeling complex BRG1-associated factor 60A
  • BRG1-associated factor 60A
  • chromatin remodeling complex BAF60A subunit
  • CRACD1
  • Rsc6p
  • subfamily d, member 1
  • SWI/SNF complex 60 kDa subunit A
  • SWI/SNF complex 60 kDa subunit
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily D member 1,60 kDa BRG-1/Brm-associated factor subunit A
  • Swp73-like protein

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) is the longer transcript and encodes the full-length isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2594
Species: Hu, Mu
Applications: WB, ChIP, IHC-P, IP, PLA
NBP2-20415
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NB100-57524
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-90017
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00006598-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-P
NBP1-90015
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00006604-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-90012
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00008815-M07
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
H00006603-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
AF2944
Species: Hu
Applications: WB, Simple Western
NBP1-88932
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24493
Species: Hu, Mu, Bv, Ch, Eq, Op, Pm
Applications: WB

Publications for SMARCD1 Lysate (NBL1-16234) (0)

There are no publications for SMARCD1 Lysate (NBL1-16234).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SMARCD1 Lysate (NBL1-16234) (0)

There are no reviews for SMARCD1 Lysate (NBL1-16234). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SMARCD1 Lysate (NBL1-16234) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SMARCD1 Products

Bioinformatics Tool for SMARCD1 Lysate (NBL1-16234)

Discover related pathways, diseases and genes to SMARCD1 Lysate (NBL1-16234). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SMARCD1 Lysate (NBL1-16234)

Discover more about diseases related to SMARCD1 Lysate (NBL1-16234).
 

Pathways for SMARCD1 Lysate (NBL1-16234)

View related products by pathway.

PTMs for SMARCD1 Lysate (NBL1-16234)

Learn more about PTMs related to SMARCD1 Lysate (NBL1-16234).
 

Research Areas for SMARCD1 Lysate (NBL1-16234)

Find related products by research area.

Blogs on SMARCD1

There are no specific blogs for SMARCD1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

SMARCD1 Antibody
NBP1-88719
ARID1A Antibody
NBP1-88932

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SMARCD1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SMARCD1