SMARCC2/BAF170 Antibody

Product Discontinued

SMARCC2/BAF170 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 350-402 of Human BAF170.
• Specificity: BAF170 (M388) detects endogenous levels of BAF170 (M388) protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMARCC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of SMARCC2/BAF170 (M388) pAb in extracts from HUVEC cells.
• Theoretical MW: 170 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SMARCC2/BAF170 Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human, Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMARCC2/BAF170 Antibody

• BAF170DKFZp313D0632
• BRG1-associated factor 170
• chromatin remodeling complex BAF170 subunit
• CRACC2
• mammalian chromatin remodeling complex BRG1-associated factor 170
• Rsc8
• subfamily c, member 2
• SWI/SNF complex 170 kDa subunit
• SWI/SNF complex subunit SMARCC2
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 2
• SWI3-like protein

Background

The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF1 or SNF2alpha) and Brg-1 (also designated SNF2 or SNF2beta) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCC2
• Entrez:
  • 6601(Human)