Home » SmarcAL1 » SmarcAL1 Antibodies » SmarcAL1 Antibody

SmarcAL1 Antibody

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: SmarcAL1 Antibody [NB100-81640] - Detection of Human SMARCAL1 on HeLa whole cell lysate using NB100-81640. SMARCAL1 was also immunoprecipitated by another rabbit anti-SMARCAL1 antibody.
Independent Antibodies: Western Blot: SmarcAL1 Antibody [NB100-81640] - Detection of Human SMARCAL1 on HeLa whole cell lysate using NB100-81640. SMARCAL1 was also immunoprecipitated by another rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

Order Details

100% Guarantee on every product

SmarcAL1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 904 and 954 of human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a-like 1 using the numbering given in entry NP_054859.2 (GeneID 50485).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCAL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 1:10-1:500
Publications
Read Publication using NB100-81640.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SmarcAL1 Antibody

  • ATP-driven annealing helicase
  • EC 3.6.1
  • EC 3.6.4.-
  • HARPSWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A-like protein 1
  • HepA-related protein
  • HHARP
  • SIOD
  • SMARCA-like protein 1
  • subfamily a-like 1
  • Sucrose nonfermenting protein 2-like 1
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Pleiotrophin/PTN
Antibody [Unconjugate ...
AF-252-PB
Pleiotrophin/PTN Antibody [Unconjugated]
(10 Publications)
Species: Hu
Applications: WB
SMARCA1 Antibody
(2H7B8)
NBP2-61879
Western Blot: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis using SMARCA1 mAb against HEK293 (1) and SMARCA1 (AA: 933-1070)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis of paraffin-embedded rectum cancer tissues using SMARCA1 mouse mAb with DAB staining.
Flow Cytometry: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis of NIH/3T3 cells using SMARCA1 mouse mAb (green) and negative control (red).
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC
Brg1 Antibody
NB100-2594
Western Blot: Brg1 Antibody [NB100-2594] - Detection of Human and Mouse BRG1/SMARCA4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BRG1/SMARCA4 antibody NB100-2594 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: Brg1 Antibody [NB100-2594] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Western Blot: Brg1 Antibody [NB100-2594] - Detection of Human and Mouse BRG1/SMARCA4 on HeLa whole cell lysate using NB100-2594.
(4 Publications)
Species: Hu, Mu
Applications: WB, ChIP, IHC, IHC-P, IP, PLA
BRM Antibody
NBP1-90015
Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP1-90015] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neuronal cells.
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Midkine Antibody
[Unconjugated]
AF-258-PB
Midkine was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-258-PB) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Midkine was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑258‑PB) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-258-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Midkine at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(4 Publications)
Species: Hu
Applications: WB, Simple Western, IHC
ZRANB3 Antibody
NBP2-55227
Immunocytochemistry/Immunofluorescence: ZRANB3 Antibody [NBP2-55227] - Staining of human cell line MCF7 shows localization to nucleus.
Species: Hu
Applications: ICC/IF
DDT Antibody
(OTI1H3)
NBP2-45677
Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of extracts (35ug) from 9 different cell lines by using DDT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDT.
Species: Hu, Mk
Applications: WB, IHC
ATRX Antibody
NBP1-83077
Western Blot: ATRX Antibody [NBP1-83077] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ATRX antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human liver shows no positivity in hepatocytes as expected.
Western Blot: ATRX Antibody [NBP1-83077] - Analysis in human cell line A-549.
(17 Publications)
Species: Hu
Applications: WB, IHC, IHC-P, KD
Myoglobin Antibody
(5A2G8)
NBP1-47364
Western Blot: Myoglobin Antibody (6H8B5) [NBP1-47364] - Analysis using Myoglobin mouse mAb against truncated Myoglobin recombinant protein(AA: 2-154).
Immunohistochemistry-Paraffin: Myoglobin Antibody (6H8B5) [NBP1-47364] - Analysis of human skeletal muscle tissue showing cytoplasmic localization using anti-Myoglobin antibody with DAB staining.
Species: Hu
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
GLS2 Antibody
NBP1-76544
Immunocytochemistry/Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.
Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.
Simple Western: GLS2 Antibody [NBP1-76544] - Simple Western lane view shows a specific band for GLS2 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P
MVK Antibody
[Unconjugated]
AF8516
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human MVK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8516) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MVK at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MVK was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human MVK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8516) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MVK was immunoprecipitated from 400 µg of HepG2 human hepatocellular carcinoma cell line and A431 human epithelial carcinoma cell line lysates using 4 µg of Rabbit Anti-Human MVK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8516) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). HepG2 lysates, A431 lysates, Rabbit IgG control buffer, or recombinant human MVK were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated MVK was detected by Western blot under reducing conditions using 0.1 µg/mL Rabbit Anti-Human MVK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8516) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.
Species: Hu
Applications: WB, Simple Western, IP, ICC
ATP7A Antibody
(S60-4)
NBP2-59376
Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Analysis of Rat Brain Membrane showing detection of ~180 kDa Copper Transporting ATPase 1 protein using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~180 kDa. Other Band(s): 250kDa.
Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP
Alpha Actinin 4
Antibody (4D10)
H00000081-M01
Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10. Analysis of ACTN4 expression in NIH/3T3.
Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of monoclonal antibody to ACTN4 on HeLa cell. Antibody concentration 20 ug/ml.
Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human lung adenocarcinoma. Antibody concentration 3 ug/ml.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
FGF basic/FGF2
[Unconjugated]
233-FB/CF
1 µg/lane of Recombinant Human FGF basic (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human FGF basic (146aa) (Catalog # 233-FB/CF) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.
(215 Publications)
Species: Hu
Albumin Antibody
NB600-41532
Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.
(38 Publications)
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Glutaminase
Antibody
NBP2-29940
Western Blot: Glutaminase Antibody [NBP2-29940] - Western blot analysis of lysates from human brain tissue and Hela cell line (from left to right), using (C-term)(Cat. # NBP2-29940). The antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Immunocytochemistry/Immunofluorescence: Glutaminase Antibody [NBP2-29940] - Fluorescent image of Hela cells stained with XAF1 (C-term)(Cat# NBP2-29940). The antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP2-29940] - Immunohistochemical analysis of paraffin-embedded H.kidney section using (C-term)(Cat# NBP2-29940). The antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC-P

Publications for SmarcAL1 Antibody (NB100-81640)(1)


Reviews for SmarcAL1 Antibody (NB100-81640) (0)

There are no reviews for SmarcAL1 Antibody (NB100-81640). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SmarcAL1 Antibody (NB100-81640) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SmarcAL1 Products

Bioinformatics Tool for SmarcAL1 Antibody (NB100-81640)

Discover related pathways, diseases and genes to SmarcAL1 Antibody (NB100-81640). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SmarcAL1 Antibody (NB100-81640)

Discover more about diseases related to SmarcAL1 Antibody (NB100-81640).
 

Pathways for SmarcAL1 Antibody (NB100-81640)

View related products by pathway.

PTMs for SmarcAL1 Antibody (NB100-81640)

Learn more about PTMs related to SmarcAL1 Antibody (NB100-81640).

Blogs on SmarcAL1

There are no specific blogs for SmarcAL1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SmarcAL1 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol SMARCAL1
Entrez
Uniprot