Smad2 Antibody

Western blot shows recombinantDrosophilaSmad2 (2 ng/lane). PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-DrosophilaSmad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Smad2 (C-terminal fragment) at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows wild-type and Smad2 null mutation Drosophila larval extracts. PVDF membrane was probed with 0.7 µg/mL of Sheep Anti-Drosophila Smad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) followed by Anti-Sheep IgG Secondary Antibody. A specific band was detected for Smad2 at the expected mobility of approximately 58 kDa, but not mutant extracts. This experiment was conducted under reducing conditions. dSmad2 null mutation is described in Peterson, AJ, et al. (2012) PLoS One 7: e36548. Image courtesy of Dr. Aidan Peterson and Dr. Michael O'Connor, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.

Smad2 was detected in wild-type and Smad2 null mutation Drosophila larvae using Sheep Anti-Drosophila Smad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) at 0.7 μg/mL. Tissue was stained using a fluorescent Anti-Sheep IgG Secondary Antibody. A single confocal image in the upper panel depicts detection of endogenous Smad2 in epidermal and central nervous system cells. The dSmad2 mutant embryo in the lower panel was stained and imaged identically and reveals background staining. dSmad2 null mutation is described in Peterson, AJ, et al. (2012) PLoS One 7: e36548. Image courtesy of Dr. Aidan Peterson and Dr. Michael O'Connor, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.

• Reactivity: Dr
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Smad2 Antibody Summary

• Immunogen: E. coli-derived recombinant Drosophila Smad2
  Gly262-Ser486
  Accession # O96660
• Specificity: Detects Drosophila Smad2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Smad1 is observed, and less than 1% cross-reactivity with rhSmad2, rhSmad3, and rhSmad5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SMAD2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.1-0.7 ug/mL
  • Western Blot 0.1-0.7 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad2 Antibody

• hMAD-2
• hSMAD2
• JV18
• JV18-1
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2
• MADH2MGC34440
• MADR2
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smox

Background

Smad2 (SMAll body plus Mothers Against Decapentaplegic; also SmoX [Smad on Chr X]) is a 54 kDa (predicted) member of the receptor regulated Smad family of proteins. It is a downstream component of the Drosophila activin/TGF beta signaling pathway, and appears to play a key role in cell proliferation. Analogous to the system in vertebrates, stimulation of the fruitfly activin type I receptor (baboon):type II receptor (punt) complex results in dSmad2 (Drosophila Smad2) phosphorylation and subsequent interaction with dSmad4/Medea. As in vertebrates, the Drosophila Samd2/Smad4 complex enters the nucleus and interacts with a transcriptional cofactor(s) termed TGIF. At this point, the vertebrate:invertebrate systems diverge over the nature of the TGIF cofactor(s); vertebrate TGIF is a corepressor, while invertebrate TGIF (dTGIF) is a coactivator. Drosophila Smad2 is 486 amino acids (aa) in length. It contains two Mad homology domains (aa 7-130 and 285-475), the former of which participates in DNA-binding. Over aa 262-486, Drosophila Smad2 shares 70% aa sequence identity with both mouse and human Smad2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad2 Antibody (AF7948)(2)

Publications using AF7948

• Jun Zhou, Yasamin Dabiri, Rodrigo A. Gama-Brambila, Shahrouz Ghafoory, Mukaddes Altinbay, Arianeb Mehrabi et al. pVHL-mediated SMAD3 degradation suppresses TGF-beta signaling Journal of Cell Biology 2022-01-03 [PMID: 34860252]
• Aidan J. Peterson, Michael B. O’Connor Strategies for exploring TGF-beta signaling in Drosophila Methods 2014-06-15 [PMID: 24680699]

Bioinformatics

• Gene Symbol: SMAD2
• Uniprot:
  • O96660(Drosophila)