Smad2 Antibody (5G7)

Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Smad2 Antibody (5G7) [NBP2-37580] - Immunofluorescence analysis of U251 cells using SMAD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using SMAD2 mouse mAb with DAB staining.

Flow Cytometry: Smad2 Antibody (5G7) [NBP2-37580] - Flow cytometric analysis of NIH/3T3 cells using SMAD2 mouse mAb (blue) and negative control (red).

Western Blot: Smad2 Antibody (5G7) [NBP2-37580] - Western blot analysis using SMAD2 mAb against human SMAD2 (AA: 20-254) recombinant protein. (Expected MW is 52.2 kDa)

Immunohistochemistry: Smad2 Antibody (5G7) [NBP2-37580] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using SMAD2 mouse mAb with DAB staining.

ELISA: Smad2 Antibody (5G7) [NBP2-37580] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 5G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Smad2 Antibody (5G7) Summary

• Description: Novus Biologicals Mouse Smad2 Antibody (5G7) (NBP2-37580) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Smad2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMAD2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Smad2 Antibody (5G7)

• hMAD-2
• hSMAD2
• JV18
• JV18-1
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2
• MADH2MGC34440
• MADR2
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smox

Background

Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMAD2