Smad2 Antibody (376520) [Unconjugated]

Smad2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Smad2 Monoclonal Antibody (Catalog # MAB4037) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 376520
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Smad2 Antibody (376520) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Smad2
  Lys20-Thr108
  Accession # Q15796
• Specificity: Detects human Smad2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Smad1, 3, 4, 5, 6, 7, or 8 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMAD2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad2 Antibody (376520) [Unconjugated]

• hMAD-2
• hSMAD2
• JV18
• JV18-1
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2
• MADH2MGC34440
• MADR2
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smox

Background

Smad2 is a 52 kDa TGF-beta receptor-regulated member of the Smad family. It is expressed most highly in skeletal muscle, heart and placenta. Receptor binding of TGF-beta causes C-terminal phosphorylation of Smads 2 and 3. Smad is released from cytoplasmic anchoring, complexes with Smad4 and accumulates in the nucleus. After regulating expression, Smad2 is dephosphorylated and recycled. The 467 aa human Smad2 shows only two aa differences with mouse Smad2. About 10% of Smad2 expressed as a short, high-activity isoform missing aa 80‑108 within the MH1 domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMAD2
• Uniprot:
  • Q15796(Human)