Smad2 Antibody (376520) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 376520
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Smad2 Antibody (376520) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Smad2
  Lys20-Thr108
  Accession # Q15796
• Specificity: Detects human Smad2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Smad1, 3, 4, 5, 6, 7, or 8 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad2 Antibody (376520) [Alexa Fluor® 488]

• hMAD-2
• hSMAD2
• JV18
• JV18-1
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2
• MADH2MGC34440
• MADR2
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smox

Background

Smad2 is a 52 kDa TGF-beta receptor-regulated member of the Smad family. It is expressed most highly in skeletal muscle, heart and placenta. Receptor binding of TGF-beta causes C-terminal phosphorylation of Smads 2 and 3. Smad is released from cytoplasmic anchoring, complexes with Smad4 and accumulates in the nucleus. After regulating expression, Smad2 is dephosphorylated and recycled. The 467 aa human Smad2 shows only two aa differences with mouse Smad2. About 10% of Smad2 expressed as a short, high-activity isoform missing aa 80‑108 within the MH1 domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.