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Recombinant Human SLP-76/LCP2 His Protein

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SDS-Page: Recombinant Human SLP-76/LCP2 Protein [NBP2-61206] - 15 % SDS-PAGE (3ug)
SDS-Page: Recombinant Human SLP-76/LCP2 Protein [NBP2-61206] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

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Recombinant Human SLP-76/LCP2 His Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-533 of Human SLP-76/LCP2

Source: Baculovirus

Amino Acid Sequence: ADPMALRNVP FRSEVLGWDP DSLADYFKKL NYKDCEKAVK KYHIDGARFL NLTENDIQKF PKLRVPILSK LSQEINKNEE RRSIFTRKPQ VPRFPEETES HEEDNGGWSS FEEDDYESPN DDQDGEDDGD YESPNEEEEA PVEDDADYEP PPSNDEEALQ NSILPAKPFP NSNSMYIDRP PSGKTPQQPP VPPQRPMAAL PPPPAGRNHS PLPPPQTNHE EPSRSRNHKT AKLPAPSIDR STKPPLDRSL APFDREPFTL GKKPPFSDKP SIPAGRSLGE HLPKIQKPPL PPTTERHERS SPLPGKKPPV PKHGWGPDRR ENDEDDVHQR PLPQPALLPM SSNTFPSRST KPSPMNPLPS SHMPGAFSES NSSFPQSASL PPYFSQGPSN RPPIRAEGRN FPLPLPNKPR PPSPAEEENS LNEEWYVSYI TRPEAEAALR KINQDGTFLV RDSSKKTTTN PYVLMVLYKD KVYNIQIRYQ KESQVYLLGT GLRGKEDFLS VSDIIDYFRK MPLLLIDGKN RGSRYQCTLT HAAGYPHHHH HH
Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Gene
LCP2
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human SLP-76/LCP2 His Protein

  • LCP2
  • lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of76kDa)
  • lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of76kD)
  • lymphocyte cytosolic protein 2
  • SH2 domain-containing leukocyte protein of 76 kDa
  • SH2 domain-containing leukocyte protein of 76kD
  • SLP-76 tyrosine phosphoprotein
  • SLP76
  • SLP-76
  • SLP7676 kDa tyrosine phosphoprotein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Immunohistochemistry-Paraffin: LAT Antibody [NBP2-27104] - FFPE human small intestine stained with LAT antibody at 10 ug/mL.
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Western Blot: ORC3L Antibody [NB100-796] - Staining of HeLa lysate (RIPA buffer, 1.4E5 cells per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ORC3L Antibody [NB100-796] - Staining of paraffin embedded Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western Blot: SPNS1 Antibody [NBP1-92440] - Analysis in human cell line HEK 293.
Immunohistochemistry-Paraffin: SPNS1 Antibody [NBP1-92440] - Staining of human lung shows strong granular cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: SPNS1 Antibody [NBP1-92440] - Staining of human lung, pancreas, placenta and testis using Anti-SPNS1 antibody NBP1-92440 (A) shows similar protein distribution across tissues to independent antibody NBP1-92441 (B).
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Immunohistochemistry-Paraffin: ZAP70 Antibody (3D2A4) [NB110-60490] - Analysis of human lymph tumor, showing cytoplasmic localization using ZAP70 mouse mAb with DAB staining
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Western Blot: GRAP2 Antibody (1G2) [NBP2-00764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRAP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRAP2.
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Vav-1 [p Tyr160]
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Vav‑1 phosphorylated at Y160 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Vav‑1 (Y160) Monoclonal Antibody (Catalog # MAB37861) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Src [p Tyr419]
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Immunocytochemistry/Immunofluorescence: GRB2 Antibody (11-B11) [NBP2-80405] - Staining GRB2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: GRB2 Antibody (11-B11) [NBP2-80405] - Staining GRB2 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: GRB2 Antibody (11-B11) [NBP2-80405] - Analysis of human colon cancer tissue using GRB2 antibody. Counter stained with hematoxylin.
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Lck [p Tyr394]
Antibody (755103)
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Lck&amp;nbsp;phosphorylated at Y394 was detected in immersion fixed Jurkat human acute T cell leukemia cells treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Lck (Y394) Monoclonal Antibody (Catalog # MAB7500) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1&amp;nbsp;mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1&amp;nbsp;µg/mL of Mouse Anti-Human Phospho-Lck (Y394) Monoclonal Antibody (Catalog # MAB7500) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog&amp;nbsp;# HAF018). A specific band was detected for Phospho-Lck (Y394) at approximately 56&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group&amp;nbsp;1.
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Western Blot: CBL Antibody (3B12) [NBP2-37574] - Western blot analysis using C-CBL mAb against human C-CBL (AA: 684-865) recombinant protein. (Expected MW is 44.9 kDa)
Western Blot: CBL Antibody (3B12) [NBP2-37574] - Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
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NCK1 Antibody
(JU41-80)
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Immunocytochemistry/Immunofluorescence: NCK1 Antibody (JU41-80) [NBP2-75585] - Staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: NCK1 Antibody (JU41-80) [NBP2-75585] - Staining NCK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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EphB2 Antibody
[Unconjugated]
AF467
Simple Western lane view shows lysates of COLO&amp;nbsp;205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for EphB2 at approximately 139 and 146&amp;nbsp;kDa (as indicated) using 20&amp;nbsp;µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog&amp;nbsp;# 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog&amp;nbsp;# 467-B2). PVDF Membrane was probed with 0.1&amp;nbsp;µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). A specific band was detected for EphB2 at approximately 120&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF467) at 5&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(9 Reviews)
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Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The&amp;nbsp;ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog&amp;nbsp;# 140-B1).
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Applications: BA
Recombinant Human
SLP-76/LCP2 His Prote ...
NBP2-61206
Recombinant Human SLP-76/LCP2 His Protein
Species: Hu
Applications: PAGE

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Simple Western lane view shows lysates of COLO&nbsp;205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2&nbsp;mg/mL. A specific band was detected for EphB2 at approximately 139 and 146&nbsp;kDa (as indicated) using 20&nbsp;µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Bioinformatics

Gene Symbol LCP2