Recombinant Human SLP-76/LCP2 His Protein

SDS-Page: Recombinant Human SLP-76/LCP2 Protein [NBP2-61206] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human SLP-76/LCP2 His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-533 of Human SLP-76/LCP2
  
  

  Source: Baculovirus

  Amino Acid Sequence: ADPMALRNVP FRSEVLGWDP DSLADYFKKL NYKDCEKAVK KYHIDGARFL NLTENDIQKF PKLRVPILSK LSQEINKNEE RRSIFTRKPQ VPRFPEETES HEEDNGGWSS FEEDDYESPN DDQDGEDDGD YESPNEEEEA PVEDDADYEP PPSNDEEALQ NSILPAKPFP NSNSMYIDRP PSGKTPQQPP VPPQRPMAAL PPPPAGRNHS PLPPPQTNHE EPSRSRNHKT AKLPAPSIDR STKPPLDRSL APFDREPFTL GKKPPFSDKP SIPAGRSLGE HLPKIQKPPL PPTTERHERS SPLPGKKPPV PKHGWGPDRR ENDEDDVHQR PLPQPALLPM SSNTFPSRST KPSPMNPLPS SHMPGAFSES NSSFPQSASL PPYFSQGPSN RPPIRAEGRN FPLPLPNKPR PPSPAEEENS LNEEWYVSYI TRPEAEAALR KINQDGTFLV RDSSKKTTTN PYVLMVLYKD KVYNIQIRYQ KESQVYLLGT GLRGKEDFLS VSDIIDYFRK MPLLLIDGKN RGSRYQCTLT HAAGYPHHHH HH

• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: LCP2
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 61.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human SLP-76/LCP2 His Protein

• LCP2
• lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of76kDa)
• lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of76kD)
• lymphocyte cytosolic protein 2
• SH2 domain-containing leukocyte protein of 76 kDa
• SH2 domain-containing leukocyte protein of 76kD
• SLP-76 tyrosine phosphoprotein
• SLP76
• SLP-76
• SLP7676 kDa tyrosine phosphoprotein

Background

SLP76 (SH2 domain containing leukocyte protein of 76 kDa) is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: LCP2