SLITRK1 Antibody (327808)

• Reactivity: Hu
• Applications: WB
• Clone: 327808
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SLITRK1 Antibody (327808) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SLITRK1
  Ala16-Ser616
  Accession # Q96PX8
• Specificity: Detects human SLITRK1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) SLITRK2, rhSLITRK4, or rhSLITRK5 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLITRK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLITRK1 Antibody (327808)

• FLJ54428
• KIAA1910KIAA0918
• leucine rich repeat containing 12
• Leucine-rich repeat-containing protein 12
• LRRC12
• RP11-395N17.1
• SLIT and NTRK-like family, member 1
• SLIT and NTRK-like protein 1
• slit and trk like gene 1
• SLITRK1
• TTM

Background

SLITRK1 (Slit and Trk-like family member 1) is a charter member of the SLITRK family of proteins (1, 2). This family includes six members, all of which contain a slit-like extracellular region, and all of which (save for SLITRK1) contain a Trk-like cytoplasmic region (3). Human SLITRK1 is a type I transmembrane protein 696 amino acids (aa) in length. It contains a 17 aa signal sequence, a 605 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in slit. Each LRD (aa 57‑200; aa 374‑517) contains six leucine-rich repeats (LRRs), followed by a Cys-rich, C-terminal LRR (aa 212‑262; aa 529‑579). The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction (4). There are multiple, single aa mutations in the ECD. Their significance is unclear (5). One truncating frameshift mutation is known that shows a unique 27 aa substitution after Arg 421 (6). This 448 aa form is associated with Tourette’s Syndrome. As noted, the cytoplasmic region is unique among SLITRK family members. It contains no known signaling motifs, which is in contrast to SLITRK2‑6 which show TrkA-like tyrosine phosphorylation sites. This difference is reflected in their function. SLITRK1 is found in adult neurons of the cerebrum, thalamus and hippocampus. It induces neurite outgrowth whereas SLITRK2‑6 block neurite extension (3). Human SLITRK1 shares 99%, 98% and 99% aa sequence identity with canine, mouse and bovine SLITRK1, respectively.

1. Aruga, J. et al. (2003) Gene 315:87.
2. Nagase, T. et al. (2001) DNA Res. 8:179.
3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
4. Enkhbayar, P. et al. (2003) Proteins 54:394.
5. Zuchner, S. et al. (2006) Mol. Psychiat. 11:888.
6. Abelson, J.F. et al. (2005) Science 310:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLITRK1
• Uniprot:
  • Q96PX8()