Slit2 Antibody

Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Slit2 Antibody Summary

• Description: Novus Biologicals Goat Slit2 Antibody (NB100-40807) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Slit2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence DDCQDNKCKNGAH corresponding to internal region according to NP_004778.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLIT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 8 ug/ml
  • Immunohistochemistry-Paraffin 4 - 8 ug/ml
  • Peptide ELISA Detection limit 1:1000
• Application Notes: IHC-P: Human Spinal Cord shows staining of the cellular membrane in the bundles of sensory fibres.
• Control:

  Slit2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Slit2 Antibody

• FLJ14420
• SLIL3
• slit (Drosophila) homolog 2
• slit homolog 2 (Drosophila)
• slit homolog 2 protein
• Slit2
• Slit-2SLIL3

Background

Slit proteins are ligands at the Roundabout (Robo) receptors and act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. A number of cleavage products are reported in the literature for Slit2 protiens (following alternate splicing). The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Slit2 Antibody (NB100-40807)(1)

Publication using NB100-40807

• Lin L, Rao Y, Isacson O. Netrin-1 and slit-2 regulate and direct neurite growth of ventral midbrain dopaminergic neurons. Mol Cell Neurosci 2005-03-01 [PMID: 15737744]

Bioinformatics

• Gene Symbol: SLIT2
• Entrez:
  • 9353(Human)
  • 20563(Mouse)
  • 360272(Rat)
• Uniprot:
  • O94813()