Slit2 Antibody [FITC]

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: FITC

Slit2 Antibody [FITC] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Slit2
  Gln26-Gln900
  Accession # Q9R1B9
• Specificity: Detects mouse Slit2 in direct ELISAs. In direct ELISAs, less than 50% cross-reactivity with recombinant human (rh) Slit2, rhSlit1 and recombinant mouse (rm) Slit1 is observed, less than 10% cross-reactivity with rmSlit3 and rhSlit3 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SLIT2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Slit2 Antibody [FITC]

• FLJ14420
• SLIL3
• slit (Drosophila) homolog 2
• slit homolog 2 (Drosophila)
• slit homolog 2 protein
• Slit2
• Slit-2SLIL3

Background

Slit proteins are ligands at the Roundabout (Robo) receptors and act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. A number of cleavage products are reported in the literature for Slit2 protiens (following alternate splicing). The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLIT2