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![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Analysis of SLC6A4 expression in mouse lung.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Western-Blot-H00006532-D01P-img0003.jpg "Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Analysis of SLC6A4 expression in mouse lung.")
![Flow Cytometry: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Fixed and permeabilized mouse mast cells were stained for 1 hour in 4C then stained with PE secondary and ran on FACS. Image from verified customer review.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Flow-Cytometry-H00006532-D01P-img0006.jpg)
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Analysis of SLC6A4 expression in transfected 293T cell line by SLC6A4 polyclonal antibody. Lane 1: SLC6A4 transfected lysate (70.30 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Western-Blot-H00006532-D01P-img0004.jpg)
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NBP3-05546] - Different tissue and cell lysates using NBP3-05546 at a dilution of 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. The prominent band at ~68kDa corresponds to SLC6A4 protein, and lower bands are likely are proteolytic products of SLC6A4. [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SLC6A4 cDNA. The bands between 50-75kDa mark in the transfected cells demonstrate overexpression of SLC6A4, and the band at about 150kDa presumably corresponds to an aggregated form of SLC6A4. The blot was probed with mouse mAb to GAPDH at a dilution of 1:5,000, in red, which reveals the single band at ~37kDa in both transfected and non-transfected cells. Note that HEK293 cells express endogenously (7).](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Western-Blot-NBP3-05546-img0002.jpg "Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NBP3-05546] - Different tissue and cell lysates using NBP3-05546 at a dilution of 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. The prominent band at ~68kDa corresponds to SLC6A4 protein, and lower bands are likely are proteolytic products of SLC6A4. [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SLC6A4 cDNA. The bands between 50-75kDa mark in the transfected cells demonstrate overexpression of SLC6A4, and the band at about 150kDa presumably corresponds to an aggregated form of SLC6A4. The blot was probed with mouse mAb to GAPDH at a dilution of 1:5,000, in red, which reveals the single band at ~37kDa in both transfected and non-transfected cells. Note that HEK293 cells express endogenously (7).")
![Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NBP3-05546] - Immunofluorescent analysis of a rat brain section stained with SLC6A4/5-HTTLPR/Serotonin transporter Antibody in green and counterstained with chicken polyclonal antibody to tyrosine hydroxylase in red. The axons of serotoninergic neurons course throughout the section but are clearly distinct from the chatacholinergic processes and cell bodies revealed with the TH antibody. The blue stain reveals DNA in cell nuclei.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05546-img0001.jpg)
![ELISA: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (2A9) [H00006532-M06] - Detection limit for recombinant GST tagged SLC6A4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-(2A9)-ELISA-H00006532-M06-img0001.jpg "ELISA: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (2A9) [H00006532-M06] - Detection limit for recombinant GST tagged SLC6A4 is approximately 0.3ng/ml as a capture antibody.")
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [NBP3-15668] - Western blot analysis of extracts of various cell lines, using SLC6A4/5-HTTLPR/Serotonin transporter antibody (NBP3-15668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-(5T7M2)-Western-Blot-NBP3-15668-img0002.jpg "Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [NBP3-15668] - Western blot analysis of extracts of various cell lines, using SLC6A4/5-HTTLPR/Serotonin transporter antibody (NBP3-15668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [NBP3-15668] - Immunofluorescence analysis of human liver cancer using SLC6A4/5-HTTLPR/Serotonin transporter Rabbit mAb (NBP3-15668) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-(5T7M2)-Immunocytochemistry-Immunofluorescence-NBP3-15668-img0001.jpg)
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [NBP3-15668] - Western blot analysis of extracts of various cell lines, using SLC6A4/5-HTTLPR/Serotonin transporter antibody (NBP3-15668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-(5T7M2)-Western-Blot-NBP3-15668-img0003.jpg)
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Human Colon lysate, 35 ug protein in RIPA buffer. Antibody at 2 ug/mL. Detection by chemiluminescence.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Western-Blot-NB100-1892-img0004.jpg "Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Human Colon lysate, 35 ug protein in RIPA buffer. Antibody at 2 ug/mL. Detection by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1892-img0005.jpg)
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter [p Thr276] Antibody [NBP2-60778] - Western blot of rat mid brain membrane lysate showing specific immunolabeling of the ~76 kDa SERT protein phosphorylated at Thr276 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-[p-Thr276]-Antibody-Western-Blot-NBP2-60778-img0001.jpg "Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter [p Thr276] Antibody [NBP2-60778] - Western blot of rat mid brain membrane lysate showing specific immunolabeling of the ~76 kDa SERT protein phosphorylated at Thr276 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).")
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) [NBP3-15198] - Positive WB detected in: U-87 whole cell lysate, 293T whole cell lysate, U-251 whole cell lysate, HepG2 whole cell lysate. All lanes: SLC6A4/5-HTTLPR/Serotonin transporter antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 71, 75 KDa. Observed band size: 55 KDa.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-(9G3)-Western-Blot-NBP3-15198-img0002.jpg "Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) [NBP3-15198] - Positive WB detected in: U-87 whole cell lysate, 293T whole cell lysate, U-251 whole cell lysate, HepG2 whole cell lysate. All lanes: SLC6A4/5-HTTLPR/Serotonin transporter antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 71, 75 KDa. Observed band size: 55 KDa.")
![Immunohistochemistry: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (9G3) [NBP3-15198] - IHC image of SLC6A4/5-HTTLPR/Serotonin transporter antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-(9G3)-Immunohistochemistry-NBP3-15198-img0001.jpg)
![SDS-Page: Recombinant Human SLC6A4/5-HTTLPR/Serotonin transporter Protein [H00006532-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize2/Recombinant-Human-SLC6A4-5-HTTLPR-Serotonin-transporter-Protein-SDS-Page-H00006532-P01-img0003.jpg "SDS-Page: Recombinant Human SLC6A4/5-HTTLPR/Serotonin transporter Protein [H00006532-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NBP2-57729] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus & vesicles.](http://images.novusbio.com/fullsize2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57729-img0001.jpg "Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NBP2-57729] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus & vesicles.")
![Western Blot: Serotonin transporter Overexpression Lysate (Adult Normal) [NBP2-04189] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Serotonin transporter.](http://images.novusbio.com/fullsize/Serotonin-transporter-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBP2-04189-img0001.jpg "Western Blot: Serotonin transporter Overexpression Lysate (Adult Normal) [NBP2-04189] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Serotonin transporter.")
-img0001.jpg "Rat SLC6A4/5-HTTLPR/Serotonin transporter ELISA Kit (Colorimetric) - Standard Curve Reference")