Mouse SLC6A3/DAT1 ELISA Kit (Colorimetric)

ELISA: Mouse SLC6A3/DAT1 ELISA Kit (Colorimetric) [NBP2-67272] - Samples were spiked with high concentrations of Mouse SLC6A3/DAT1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse SLC6A3/DAT1 ELISA Kit (Colorimetric) [NBP2-67272] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 7.81 - 500 pg/mL
• Sensitivity: 4.69 pg/mL

Mouse SLC6A3/DAT1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse DAT in samples. No significant cross-reactivity or interference between Mouse DAT and analogues was observed.
• Standard Curve Range: 7.81 - 500 pg/mL
• Sensitivity: 4.69 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 7.76%
• Intra-Assay: CV% < 4.7%
• Spike Recovery: 84-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SLC6A3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse SLC6A3/DAT1 ELISA Kit (Colorimetric)

• DA transporter
• DAT1
• DATDAT1Solute carrier family 6 member 3
• SLC6A3
• sodium-dependent dopamine transporter
• solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• VNTR

Background

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for SLC6A3/DAT1 ELISA Kit (NBP2-67272). (Showing 1 - 2 of 2 FAQ).

1. Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
   • Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SLC6A3