SLC6A2/NET/Noradrenaline transporter Antibody (NET-05)

Product Discontinued

SLC6A2/NET/Noradrenaline transporter Antibody (NET-05) Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
• Specificity: Specific for the ~55kDa Norepinephrine Transporter in Western blots of mouse and rat cortex homogenate. No reactivity with tissue or cells from NET knock-out mice.
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC6A2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for SLC6A2/NET/Noradrenaline transporter Antibody (NET-05)

• NAT1
• NAT1neurotransmitter transporter
• NET
• NET1sodium-dependent noradrenaline transporter
• Noradrenalin Transporter
• Norepinephrine Transporter
• SLC6A2
• SLC6A5
• solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2
• Solute carrier family 6 member 2
• solute carrier family 6 member 5

Background

Norepinephrine Transporter [NET] (or noradrenaline transporter (NAT)) is a monoamine transporter that transports the neurotransmitter noradrenaline from the synapse back to its vesicles for storage until later use. It also appears to transport the neurotransmitter dopamine in the same way, but to a lesser degree. Studies have shown a decrease in NET levels in the locus coeruleus in patients diagnosed with major depression (Klimek et al., 1997). Cocaine, amphetamines and many therapeutic antidepressants, such as the SNRIs (Serotonin-norepinephrine reuptake inhibitors) and the tricyclic antidepressants (TCAs) act to raise noradrenaline. Furthermore, deficits in the NET gene have been associated with ADHD (Kim et al., 2006).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC6A2/NET/Noradrenaline transporter Antibody (NBP1-28665)(4)

Publications using NBP1-28665

• Dixit B, Vanhoozer S, Anti N et al. Rapid enrichment of mitochondria from mammalian cell cultures using digitonin MethodsX 2021-08-26 [PMID: 34434723]
• Zerbi, V, Floriou-Servou, A Et al. Rapid Reconfiguration of the Functional Connectome after Chemogenetic Locus Coeruleus Activation. Neuron 2019-08-21 [PMID: 31227310] (IF/IHC, Mouse)
• Stout K, Bernaskova M, Miller GW et al. Bioinspired Honokiol Analogs and Their Evaluation for Activity on the Norepinephrine Transporter bioRxiv 2018-10-22 [PMID: 30287800] (WB, Human)
• Klimek V, Stockmeier C, Overholser J et al. Reduced levels of norepinephrine transporters in the locus coeruleus in major depression. J Neurosci. 1997-11-01 [PMID: 9334417]

Bioinformatics

• Gene Symbol: SLC6A2
• Entrez:
  • 282363(Bovine)
  • 20538(Mouse)
• Uniprot:
  • O55192(Mouse)