SLC26A3 Overexpression Lysate

Images

 
Western Blot: SLC26A3 Overexpression Lysate (Adult Normal) [NBL1-16077] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC26A3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SLC26A3 Overexpression Lysate Summary

Description

SLC26A3 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC206559

Accession#: NM_000111

Protein Tag: C-MYC/DDK
Gene
SLC26A3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
84.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SLC26A3 Overexpression Lysate

  • chloride anion exchanger
  • CLD
  • congenital chloride diarrhea
  • down-regulated in adenoma protein
  • DRADown-regulated in adenoma
  • Protein DRA
  • Solute carrier family 26 member 3
  • solute carrier family 26, member 3

Background

The protein encoded by this gene is a transmembrane glycoprotein that transports chloride ions across the cell membrane in exchange for bicarbonate ions. It is localized to the mucosa of the lower intestinal tract, particularly to the apical membrane of columnar epithelium and some goblet cells. The protein is essential for intestinal chloride absorption, and mutations in this gene have been associated with congenital chloride diarrhea. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SLC26A3 Lysate (NBL1-16077) (0)

There are no publications for SLC26A3 Lysate (NBL1-16077).
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Video Protocols

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Blogs on SLC26A3.

Next-Gen DNA Sequencing and SLC26A3 Research
The SLC26A3, also known as DRA (downregulated-in-adenoma) gene is a member of the sulphate anion transporter family, serving an important role in the exchange and transport of chloride, bicarbonate and sulphate ions at plasma membrane sites. We at Nov...  Read full blog post.

Antibody Therapies and the New Generation of DNA Sequencing
Our antibody database is primarily focused on protein-coding genes. Although they form only 1% of the total human genome, these important genes account for 85% of the mutations that lead to disease.DNA sequencing (defining the sequence of the 4 base...  Read full blog post.

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Bioinformatics

Gene Symbol SLC26A3