Next-Gen DNA Sequencing and SLC26A3 Research

Thu, 09/30/2010 - 04:39

The SLC26A3, also known as DRA (downregulated-in-adenoma) gene is a member of the sulphate anion transporter family, serving an important role in the exchange and transport of chloride, bicarbonate and sulphate ions at plasma membrane sites. We at Novus offer a growing selection of antibodies, recombinant proteins, lysates and RNAi for SLC26A3 within our reagent database.

To date, more than 20 mutations of SLC26A3 are known to exist. One of which is responsible for the rare autosomal recessive disorder congenital chloridorrhea, or congenital chloride diarrhea (CLD). In September 2009, next-generation DNA sequencing was used to diagnose congenital chloride diarrhea, or CLD, in a patient previously thought to be suffering from the salt-wasting disease Bartter syndrome.

Immunoperoxidase of monoclonal antibody to SLC26A3 Immunoperoxidase of monoclonal antibody to SLC26A3

This was only possible due to the advantage next-gen sequencing presents over the conventional Sanger technique: that of analyzing many hundreds of short-chain DNA sequences, rather than just one single, long chain of bases. The molecular diagnosis was confirmed with a clinical follow-up, following which next-gen sequencing SLC26A3 antibodies were used to confirm several more misdiagnosed cases of CLD. This was the first time whole exome (i.e. genome) sequencing had been used to make a genetic diagnosis in a patient.

As well as paving the way for use of whole-exome sequencing in routine clinical diagnosis, next-gen technology could also give microarray assays a serious run for their money. Like NGS, microarrays can detect thousands of genes in one assay. The technique relies on hybridization, in which fluorescently labelled protein DNA samples and antibodies bind (hybridize) on a biochip.

We at Novus Biologicals have a broad antibody database suitable for a variety of cutting edge biological assays, including in situ hybridization and similar techinques.

Novus Biologicals offers many SLC26A3 reagents for your research needs including:


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