SLC22A2/OCT2 Overexpression Lysate (Native)

Images

 
Western Blot: SLC22A2 Overexpression Lysate (Adult Normal) [NBL1-16035] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC22A2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SLC22A2/OCT2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207921 and based on accession number NM_003058. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens solute carrier family 22 (organic cation transporter), member 2 (SLC22A2), mRNA.
Gene
SLC22A2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
62.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SLC22A2/OCT2 Overexpression Lysate (Native)

  • hOCT2
  • MGC32628
  • OCT2
  • OCT2Organic cation transporter 2
  • SLC22A2
  • solute carrier family 22 (organic cation transporter), member 2
  • solute carrier family 22 member 2

Background

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. It is found primarily in the kidney, where it may mediate the first step in cation reabsorption. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-51684
Species: Hu, Mu
Applications: WB, EM, ELISA, Flow, IHC
NBP2-21584
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-83976
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-87909
Species: Hu, Rt
Applications: IHC, IHC-P
AF5486
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, CyTOF-ready, ICC
NBP2-62690
Species: Hu
Applications: IHC, IHC-P
NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
NBP2-65203
Species: Hu
NBP1-92396
Species: Hu
Applications: IHC, IHC-P
NBP2-45374
Species: Hu
Applications: WB, IHC
H00006583-A01
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-FrFl, IHC-WhMt
NBP2-42202
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NB100-1607
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87365
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-62660
Species: Hu
Applications: WB, IHC, IHC-P
NB100-80870
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, ELISA, IHC, IHC-P
H00005452-D01P
Species: Hu, Mu, Rt
Applications: WB
NBL1-16035
Species: Hu
Applications: WB

Publications for SLC22A2/OCT2 Lysate (NBL1-16035) (0)

There are no publications for SLC22A2/OCT2 Lysate (NBL1-16035).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SLC22A2/OCT2 Lysate (NBL1-16035) (0)

There are no reviews for SLC22A2/OCT2 Lysate (NBL1-16035). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SLC22A2/OCT2 Lysate (NBL1-16035) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for SLC22A2/OCT2 Lysate (NBL1-16035)

Discover related pathways, diseases and genes to SLC22A2/OCT2 Lysate (NBL1-16035). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SLC22A2/OCT2 Lysate (NBL1-16035)

Discover more about diseases related to SLC22A2/OCT2 Lysate (NBL1-16035).
 

Pathways for SLC22A2/OCT2 Lysate (NBL1-16035)

View related products by pathway.

PTMs for SLC22A2/OCT2 Lysate (NBL1-16035)

Learn more about PTMs related to SLC22A2/OCT2 Lysate (NBL1-16035).

Blogs on SLC22A2/OCT2

There are no specific blogs for SLC22A2/OCT2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

OCT4 Antibody
NB100-2379

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SLC22A2/OCT2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC22A2
Entrez