SLC12A3 [p Thr53] Antibody - Azide Free

Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)

• Reactivity: Hu, Mu, Rt, Gp, Ha
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

SLC12A3 [p Thr53] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit SLC12A3 [p Thr53] Antibody - Azide Free (NBP2-60775) is a polyclonal antibody validated for use in IHC and WB. Anti-SLC12A3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH. Accession # P59158
• Modification: p Thr53
• Specificity: Specific for endogenous levels of the ~160 kDa SLC12A3 protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.
• Predicted Species: Guinea Pig (100%), Hamster (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC12A3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:10000
  • Western Blot 1:1000-1:6000
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for SLC12A3 [p Thr53] Antibody - Azide Free

• FLJ96318
• Na-Cl symporter
• NCC
• NCCT
• SLC12A3
• solute carrier family 12 (sodium/chloride transporters), member 3
• solute carrier family 12 member 3
• thiazide-sensitive Na-Cl cotransporter
• Thiazide-sensitive sodium-chloride cotransporter
• TSC
• TSCNaCl electroneutral thiazide-sensitive cotransporter

Background

The SLC12A3 gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman s

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC12A3 Antibody (NBP2-60775)(2)

Publications using NBP2-60775

• Matsumoto T, Doi S, Nakashima A et al. Upregulation of Mineralocorticoid Receptor Contributes to Development of Salt-Sensitive Hypertension after Ischemia-Reperfusion Injury in Rats International journal of molecular sciences 2022-07-15 [PMID: 35887178] (WB, Rat)
  
  Details:
  Dilutions: 1:2500
• KL Otterpohl, BW Busselman, I Ratnayake, RG Hart, K Hart, C Evans, CL Phillips, JR Beach, P Ahrenkiel, B Molitoris, K Surendran, I Chandrasek Conditional Myh9 and Myh10 inactivation in adult mouse renal epithelium results in progressive kidney disease JCI Insight, 2020-11-05;0(0):. 2020-11-05 [PMID: 33001861]

Bioinformatics

• Gene Symbol: SLC12A3