Recombinant Human Skp1 Protein

SDS-Page: Recombinant Human Skp1 Protein [NBP1-30173] - 15% SDS-Page (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Skp1 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1- 160 of Human Skp1
  
  

  Source: E.coli

  Amino Acid Sequence: MPSIKLQSSD GEIFEVDVEI AKQSVTIKTM LEDLGMDDEG DDDPVPLPNV NAAILKKVIQ WCTHHKDDPP PPEDDENKEK RTDDIPVWDQ EFLKVDQGTL FELILAANYL DIKGLLDVTC KTVANMIKGK TPEEIRKTFN IKNDFTEEEE AQVGSTQFCL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SKP1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 50 mM NaCl, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Skp1 Protein

• Cyclin-A/CDK2-associated protein p19
• EMC19p19skp1
• MGC34403
• OCP2
• OCP2OCP-2
• OCP-IIS-phase kinase-associated protein 1A (p19A)
• Organ of Corti protein 2
• Organ of Corti protein II
• p19a
• p19Acyclin A/CDK2-associated p19
• p19Skp1
• RNA polymerase II elongation factor-like protein OCP2
• RNA polymerase II elongation factor-like protein
• SKP1
• SKP1A
• SKP1Acyclin A/CDK2-associated protein p19
• S-phase kinase-associated protein 1
• TCEB1LSIII
• Transcription elongation factor B

Background

S-phase kinase-associated protein 1, also known as SKP1, is an F-box protein which functions as a substrate recognition component of the SCF ubiquitin ligase complex. It binds to proteins containing an F-box motif, such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis. It is also involved in the control of beta-catenin levels and the activity of beta-catenin dependent TCF transcription factors. Recombinant human SKP1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Skp1 Recombinant Protein (NBP1-30173)(1)

Publication using NBP1-30173

• Frontini M, Kukalev A, Leo E et al. The CDK Subunit CKS2 Counteracts CKS1 to Control Cyclin A/CDK2 Activity in Maintaining Replicative Fidelity and Neurodevelopment Dev Cell 2012 Aug 14 [PMID: 22898779] (PEP-ELISA)

Bioinformatics

• Gene Symbol: SKP1