Skp1 Overexpression Lysate (Native)

Images

 
Western Blot: Skp1 Overexpression Lysate (Adult Normal) [NBP2-07556] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Skp1.

Product Details

Summary
Applications WB

Order Details

Skp1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_008861. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Skp1, mRNA.
Gene
SKP1

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Skp1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Skp1 Overexpression Lysate (Native)

  • Cyclin-A/CDK2-associated protein p19
  • EMC19p19skp1
  • MGC34403
  • OCP2OCP-2
  • OCP-IIS-phase kinase-associated protein 1A (p19A)
  • Organ of Corti protein 2
  • Organ of Corti protein II
  • p19Acyclin A/CDK2-associated p19
  • RNA polymerase II elongation factor-like protein OCP2
  • RNA polymerase II elongation factor-like protein
  • SKP1Acyclin A/CDK2-associated protein p19
  • S-phase kinase-associated protein 1
  • TCEB1LSIII
  • Transcription elongation factor B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-91724
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2574
Species: Hu
Applications: ICFlow
NBP1-58219
Species: Hu
Applications: WB
H00009978-P01
Species: Hu
NBP2-31361
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-81988
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NB100-91962
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB7675
Species: Hu
Applications: WB, Simple Western, ICC
NBP1-59631
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, ICC
NBP1-69571
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-87581
Species: Hu
Applications: IHC, IHC-P
H00026232-B01P
Species: Hu, Rt
Applications: WB, ELISA
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-87104
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Skp1 Lysate (NBP2-07556) (0)

There are no publications for Skp1 Lysate (NBP2-07556).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Skp1 Lysate (NBP2-07556) (0)

There are no reviews for Skp1 Lysate (NBP2-07556). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Skp1 Lysate (NBP2-07556) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Skp1 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for Skp1 Lysate (NBP2-07556)

Discover related pathways, diseases and genes to Skp1 Lysate (NBP2-07556). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Skp1 Lysate (NBP2-07556)

Discover more about diseases related to Skp1 Lysate (NBP2-07556).
 

Pathways for Skp1 Lysate (NBP2-07556)

View related products by pathway.

PTMs for Skp1 Lysate (NBP2-07556)

Learn more about PTMs related to Skp1 Lysate (NBP2-07556).
 

Research Areas for Skp1 Lysate (NBP2-07556)

Find related products by research area.

Blogs on Skp1

There are no specific blogs for Skp1, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol SKP1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-07556 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought