Skp1 Antibody (4E11)

Western Blot: Skp1 Antibody (4E11) [NBP2-37539] - Western blot analysis using SKP1 mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), RAJI (4), PC-12 (5), Cos7 (6), MCF-7 (7) and HepG2 (8) cell lysate.

Immunocytochemistry/Immunofluorescence: Skp1 Antibody (4E11) [NBP2-37539] - Immunofluorescence analysis of Hela cells using SKP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: Skp1 Antibody (4E11) [NBP2-37539] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using SKP1 mouse mAb with DAB staining.

Flow Cytometry: Skp1 Antibody (4E11) [NBP2-37539] - Flow cytometric analysis of NIH/3T3 cells using SKP1 mouse mAb (green) and negative control (red).

Western Blot: Skp1 Antibody (4E11) [NBP2-37539] - Western blot analysis using SKP1 mAb against human SKP1 (AA: 1-160) recombinant protein. (Expected MW is 43.5 kDa)

ELISA: Skp1 Antibody (4E11) [NBP2-37539] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

Skp1 Antibody (4E11) Summary

• Immunogen: Purified recombinant fragment of human SKP1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SKP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Skp1 Antibody (4E11)

• Cyclin-A/CDK2-associated protein p19
• EMC19p19skp1
• MGC34403
• OCP2
• OCP2OCP-2
• OCP-IIS-phase kinase-associated protein 1A (p19A)
• Organ of Corti protein 2
• Organ of Corti protein II
• p19a
• p19Acyclin A/CDK2-associated p19
• p19Skp1
• RNA polymerase II elongation factor-like protein OCP2
• RNA polymerase II elongation factor-like protein
• SKP1
• SKP1A
• SKP1Acyclin A/CDK2-associated protein p19
• S-phase kinase-associated protein 1
• TCEB1LSIII
• Transcription elongation factor B

Background

Skp1 encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Skp1 Antibody (NBP2-37539)(1)

Publication using NBP2-37539

• Lavie J, Lalou C, Mahfouf W et al. The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins Cell reports 2023-05-31 [PMID: 37267103] (WB, Human)

Bioinformatics

• Gene Symbol: SKP1