SIRT4 Antibody (1C8)

Images

 
Western Blot: SIRT4 Antibody (1C8) [H00023409-M03] - Analysis of SIRT4 expression in Raw 264.7.
ELISA: SIRT4 Antibody (1C8) [H00023409-M03] - Detection limit for recombinant GST tagged SIRT4 is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
1C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SIRT4 Antibody (1C8) Summary

Immunogen
SIRT4 (NP_036372, 215 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
SIRT4 - sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) (1C8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SIRT4
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 Review rated 3
using
H00023409-M03 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SIRT4 Antibody (1C8)

  • EC 2.4.2.-
  • EC 3.5.1
  • MGC130046
  • MGC130047
  • MGC57437
  • SIR2L4NAD-dependent ADP-ribosyltransferase sirtuin-4
  • sir2-like 4
  • SIR2-like protein 4
  • sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 4
  • sirtuin 4
  • sirtuin type 4

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIRT4 Antibody (H00023409-M03) (0)

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Review for SIRT4 Antibody (H00023409-M03) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00023409-M03:
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WB
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Images Ratings Applications Species Date Details
Western Blot SIRT4 H00023409-M03
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reviewed by:
Anonymous
WB Human 11/20/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman Cancer Lines whole cell lysate
SpeciesHuman
Lot08249-1C8

Blocking

Blocking DetailsBlocked 1 hr at room temp with 5% milk powder in TBS-T

Primary Anitbody

Dilution RatioIn 3% BSA in TBS-T overnight at 4 degrees with gentle shaking.

Secondary Antibody

Secondary DescriptionAffinity purified horse anti-mouse IgG (H&L) antibody conjugated to HRP
Secondary Manufacturer Cat#Cell Signaling #7076
Secondary Concentration1:2000

Details

Detection NotesUsed Kodak ImageStation 4000MM to detect after developing with Pierce ECL Western Blotting Substrate. 2 minute exposure.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SIRT4 Antibody (H00023409-M03) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SIRT4 Antibody (H00023409-M03)

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Recent Reviews

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1
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Anonymous
11/20/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SIRT4
Entrez
Uniprot