SIRT4 Antibody (1C8) - Azide and BSA Free

Western Blot: SIRT4 Antibody (1C8) [H00023409-M03] - Analysis of SIRT4 expression in Raw 264.7.

ELISA: SIRT4 Antibody (1C8) [H00023409-M03] - Detection limit for recombinant GST tagged SIRT4 is 1 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 1C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SIRT4 Antibody (1C8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SIRT4 Antibody (1C8) - Azide and BSA Free (H00023409-M03) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: SIRT4 (NP_036372.1, 215 a.a. ~ 314 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRVKEADSLLVVGSSLQVYSGYRFILTAWEKKLPIAILNIGPTRSDDLACLKLNSRCGELLPLIDPC
• Specificity: SIRT4 - sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) (1C8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRT4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SIRT4 Antibody (1C8) - Azide and BSA Free

• EC 2.4.2.-
• EC 3.5.1
• MGC130046
• MGC130047
• MGC57437
• SIR2L4
• SIR2L4NAD-dependent ADP-ribosyltransferase sirtuin-4
• sir2-like 4
• SIR2-like protein 4
• SIRT4
• sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 4
• Sirtuin 4
• sirtuin type 4

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for SIRT4 Antibody (H00023409-M03) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 11/20/2014

Summary

• Application: Western Blot
• Sample Tested: Human Cancer Lines whole cell lysate
• Species: Human
• Lot: 08249-1C8

Bioinformatics

• Gene Symbol: SIRT4
• Entrez:
  • 23409(Human)
• Uniprot:
  • NP_036372(Human)