SIRP beta 1/gamma Antibody (OX117) - Chimeric - Azide and BSA Free

Flow Cytometry: SIRP beta 1/gamma Antibody (OX117) - Chimeric [NBP2-81072] - Flow-cytometry using [NBP2-81072]. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of OX117 (NBP2-81072, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: ELISA, Flow, IP
• Clone: OX117
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

SIRP beta 1/gamma Antibody (OX117) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human SIRPgamma:CD4 fusion protein.
• Specificity: This antibody binds to human SIRP beta 1/gamma.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SIRPB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to human Signal-regulatory protein gamma, an immunoglobulin-like cell surface receptor binding CD47. It mediates cell-cell adhesion and enhances T-cell activation.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SIRP beta 1/gamma Antibody (OX117) - Chimeric - Azide and BSA Free

• CD172 antigen-like family member B
• CD172b
• signal-regulatory protein beta-1
• SIRP beta 1/gamma
• SIRP-BETA-1

Background

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRPB1