SIRP alpha/CD172a Antibody (602407) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: ELISA
• Clone: 602407
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

SIRP alpha/CD172a Antibody (602407) [Alexa Fluor® 532] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human SIRPα/CD172a
  Gly27-Asn370
  Accession # P78324
• Specificity: Detects human SIRP alpha /CD172a in ELISA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRP alpha/CD172a Antibody (602407) [Alexa Fluor® 532]

• BIT
• BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs
• Brain Ig-like molecule with tyrosine-based activation motifs
• CD172 antigen-like family member A
• CD172a antigen
• CD172a
• Inhibitory receptor SHPS-1
• Macrophage fusion receptor
• MFR
• MFRtyrosine phosphatase SHP substrate 1
• MyD-1 antigen
• MYD1
• MYD-1
• P84
• protein tyrosine phosphatase, non-receptor type substrate 1
• PTPNS1
• SHP substrate 1
• SHPS1
• SHPS-1
• SHPS1CD172A
• signal-regulatory protein alpha
• Signal-regulatory protein alpha-1
• Signal-regulatory protein alpha-2
• Signal-regulatory protein alpha-3
• SIRP alpha
• SIRPA
• SIRPalpha
• Sirp-alpha-1
• SIRPalpha2
• Sirp-alpha-2
• Sirp-alpha-3
• SIRPtyrosine-protein phosphatase non-receptor type substrate 1

Background

SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins.  It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts.  SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis.  It binds to CD47 and likely other ligands.  Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein.  It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347).  Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases.  There is one alternative start site at Met102 plus a four aa insertion after Gln421.  Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.