Sin1/MAPKAP1 Antibody (752219) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB
• Clone: 752219
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Sin1/MAPKAP1 Antibody (752219) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human Sin1/MAPKAP1
  Lys408-Gln522
  Accession # Q9BPZ7
• Specificity: Detects human Sin1/MAPKAP1 in ELISA.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPKAP1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Sin1/MAPKAP1 Antibody (752219) [CoraFluor™ 1]

• JC310
• MAPKAP1
• MEKK2-interacting protein 1
• MGC2745
• MIP1
• MIP1SIN1SIN1b
• Mitogen-activated protein kinase 2-associated protein 1
• mitogen-activated protein kinase associated protein 1
• mSIN1
• ras inhibitor MGC2745
• SAPK-interacting protein 1
• Sin1
• SIN1g
• stress-activated map kinase interacting protein 1
• Stress-activated map kinase-interacting protein 1
• stress-activated protein kinase-interacting 1
• target of rapamycin complex 2 subunit MAPKAP1
• TORC2 subunit MAPKAP1

Background

Sin1 is a novel component of the eukaryotic SAPK pathway. Sin1 and the H3 and H4 histones interact genetically, with the C terminus of Sin1 physically associating with components of the SWI-SNF complex. This interaction is blocked by the N-terminal half of the protein in full length Sin1. It has been proposed that Sin1 acts as a regulatable bridge between the SWI-SNF complex and the nucleosome Sin1 is also required for effective transcription via the AP-1 factor Pap1, but does not prevent its nuclear translocation. Sin1 is a novel component of the eukaryotic SAPK pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPKAP1