SIL1 Overexpression Lysate (Native)

Images

 
Western Blot: SIL1 Overexpression Lysate (Adult Normal) [NBL1-15966] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIL1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SIL1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC211850 and based on accession number NM_022464. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae) (SIL1), transcript variant 2, mRNA.
Gene
SIL1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SIL1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SIL1 Overexpression Lysate (Native)

  • BAPMarinesco-Sjogren syndrome
  • BiP-associated protein
  • MSS
  • nucleotide exchange factor SIL1
  • SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
  • ULG5

Background

This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an exon in the 5'UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1419
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
NB100-56552
Species: Hu
Applications: WB
NBP2-35213
Species: Hu
NB120-7164
Species: Hu, Mu, Rt, Fi
Applications: ELISA, ICC/IF, IHC-Fr, MiAr
NB300-164
Species: Hu, Po, Ca, Rt(-)
Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP
NB100-621
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP1-86713
Species: Hu
Applications: IHC, IHC-P
NB100-56505
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
NBP1-89942
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
H00001806-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-32433
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
AF2535
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
H00003845-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-329
Species: Hu
Applications: WB, IHC, IP, PLA, RIA
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2009
Species: Hu
Applications: WB, IHC
NBL1-15966
Species: Hu
Applications: WB

Publications for SIL1 Lysate (NBL1-15966) (0)

There are no publications for SIL1 Lysate (NBL1-15966).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SIL1 Lysate (NBL1-15966) (0)

There are no reviews for SIL1 Lysate (NBL1-15966). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SIL1 Lysate (NBL1-15966) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SIL1 Products

Bioinformatics Tool for SIL1 Lysate (NBL1-15966)

Discover related pathways, diseases and genes to SIL1 Lysate (NBL1-15966). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SIL1 Lysate (NBL1-15966)

Discover more about diseases related to SIL1 Lysate (NBL1-15966).
 

Pathways for SIL1 Lysate (NBL1-15966)

View related products by pathway.

PTMs for SIL1 Lysate (NBL1-15966)

Learn more about PTMs related to SIL1 Lysate (NBL1-15966).

Blogs on SIL1

There are no specific blogs for SIL1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

p19ARF Antibody
NB200-106

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SIL1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SIL1