Siglec-6/CD327 Antibody

Western Blot: Siglec-6/CD327 Antibody [NBP1-49856] - (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Siglec-6/CD327 Antibody Summary

• Immunogen: Peptide with sequence C-KWMKYGYTSSK corresponding to internal region according to NP_001236.4, NP_942142.3, NP_942143.3.
• Epitope: KWMKYGYTSSK - internal region
• Specificity: This antibody is expected to recognize all reported isoforms (NP_001236.4; NP_942142.3; NP_942143.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIGLEC6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 60 kDa band and 35 kDa band in human placenta lysates. These bands correspond to earlier findings with different antibodies from other sources. This protein has a calculated MW of 49.9 kDa band according to NP_001236.4.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Siglec-6/CD327 Antibody

• CD327 antigen
• CD327
• CD33 antigen-like 1
• CD33L1
• CD33L1OBBP1CD327
• CD33L2
• CD33LCDW327
• CDw327
• OB-BP1
• Obesity-binding protein 1
• sialic acid binding Ig-like lectin 6
• sialic acid-binding Ig-like lectin 6
• Siglec6
• Siglec-6

Background

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linkedsialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cellsurface

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-6/CD327 Antibody (NBP1-49856)(1)

Publication using NBP1-49856

• Winn VD, Gormley M, Paquet AC et al. Severe preeclampsia-related changes in gene expression at the maternal-fetal interface include sialic acid-binding immunoglobulin-like lectin-6 and pappalysin-2. Endocrinology 2009-01-01 [PMID: 18818296]

Bioinformatics

• Gene Symbol: SIGLEC6
• Entrez:
  • 946(Human)
• Uniprot:
  • NP_001236.4(Human)