Siglec-5/Siglec-14 Antibodies

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." alt="Human blood derived monocytes were stained with Mouse Anti-Human Siglec-5/14 Monoclonal Antibody (Catalog # MAB10721, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-5/14 Mono-clonal Antibody (Catalog # MAB10721). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_lightbox"> 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-5/14 Mono-clonal Antibody (Catalog # MAB10721). The ND₅₀ is typically 0.04-0.2 µg/mL." class="big_thumb" />

1072-SL) was serially diluted and captured by Mouse Anti-Human Siglec‑5/Siglec‑14 Monoclonal Antibody (Catalog # MAB10721) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Siglec‑5/Siglec‑14 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 1072-SL) was serially diluted and captured by Mouse Anti-Human Siglec‑5/Siglec‑14 Monoclonal Antibody (Catalog # MAB10721) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Siglec‑5/Siglec‑14 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec‑5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Human Siglec‑5/14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1072). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_lightbox"> 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec‑5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Human Siglec‑5/14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1072). The ND₅₀ is typically 0.5-2.0 µg/mL." alt="Recombinant Human Siglec‑5 Fc Chimera (Catalog # 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec‑5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Human Siglec‑5/14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1072). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox"> AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal centers. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal centers. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

1072-SL) was serially diluted and captured by Mouse Anti-Human Siglec‑5/Siglec‑14 Monoclonal Antibody (Catalog # MAB10721) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Siglec‑5/Siglec‑14 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1072-SL) was serially diluted and captured by Mouse Anti-Human Siglec‑5/Siglec‑14 Monoclonal Antibody (Catalog # MAB10721) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Siglec‑5/Siglec‑14 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Siglec‑5/CD170 Fc Chimera (Catalog # 1072-SL) was serially diluted and captured by Mouse Anti-Human Siglec‑5/Siglec‑14 Monoclonal Antibody (Catalog # MAB10721) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Siglec‑5/Siglec‑14 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />