Siglec-5/Siglec-14 Antibody (194111) [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA(Det)
Clone
194111
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

Siglec-5/Siglec-14 Antibody (194111) [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/Siglec‑14
Lys18-Thr434
Accession # O15389
Specificity
Detects human Siglec‑5/Siglec‑14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC5
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human Siglec-5/Siglec-14 Antibody (Catalog # MAB10721)
ELISA Detection: Human Siglec-5/Siglec-14 Biotinylated Antibody (Catalog # BAM10722)
Standard: Recombinant Human Siglec-5/CD170 Fc Chimera (Catalog # 1072-SL)
Readout System
Publications
Read Publication using
BAM10722 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-5/Siglec-14 Antibody (194111) [Biotin]

  • Siglec-5/Siglec-14

Background

Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-5/Siglec-14 Antibody (BAM10722)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


Filter By Application
ICC
(1)
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Filter By Species
Human
(1)
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAM10722

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Bioinformatics

Gene Symbol SIGLEC5
Uniprot