Human Siglec-3/CD33 ELISA Kit (Colorimetric)

Standard Curve Reference.

Samples were spiked with high concentrations of Human Siglec-3/CD33, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.1 ng/mL

Human Siglec-3/CD33 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 3 h 30 min
• Specificity: This kit recognizes Human Siglec-3/CD33 in samples. No significant cross-reactivity or interference between Human Siglec-3/CD33 and analogues was observed
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.1 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10
• Intra-Assay: CV% < 10
• Spike Recovery: 80 - 120%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CD33

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Siglec-3/CD33 ELISA Kit (Colorimetric)

• CD33 antigen (gp67)
• CD33 antigen
• CD33 molecule
• CD33
• FLJ00391
• gp67
• myeloid cell surface antigen CD33
• p67
• sialic acid binding Ig-like lectin 3
• Sialic acid-binding Ig-like lectin 3
• Siglec3
• Siglec-3
• SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Siglec-3/CD33 ELISA Kit (NBP3-49937). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: CD33