Siglec-2/CD22 Antibody (S-HCL-1) - BSA Free

Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.

Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Separation of human CD22 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.

Western Blot: Mouse Monoclonal Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - The reactivity of S-HCL-1 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Ramos, Daudi, and Jurkat cell lines, mixed and heated (100C, 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG2b monoclonal antibody S-HCL-1 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green).CD22 was detected at ~110-125 kDa in Daudi cell line.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: S-HCL-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Siglec-2/CD22 Antibody (S-HCL-1) - BSA Free Summary

• Additional Information: Clone S-HCL-1 was used by HLDA to establish CD designation.
• Immunogen: Whole hairy cell leukemia cells and membrane preparation
• Specificity: The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD22
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Siglec-2/CD22 Antibody (S-HCL-1) - BSA Free

• B-cell receptor CD22
• BL-CAM
• B-lymphocyte cell adhesion molecule
• CD22 antigenMGC130020
• CD22 molecule
• CD22
• sialic acid binding Ig-like lectin 2
• Sialic acid-binding Ig-like lectin 2
• Siglec2
• Siglec-2
• SIGLEC2FLJ22814
• T-cell surface antigen Leu-14

Background

CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes. CD22 has at least two isoforms (alpha and beta) produced by alternate splicing. The protein exists predominantly as a monomer of the beta isoform (95 kDa). It is also found as a heterodimer of the beta and alpha isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Siglec-2/CD22 Antibody (NBP3-09167). (Showing 1 - 1 of 1 FAQ).

1. I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?
   • We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

Bioinformatics

• Gene Symbol: CD22