Siglec-11 Antibody - BSA Free

Western Blot: Siglec-11 Antibody [NBP1-77025] - HepG2 cell lysate with Siglec11 antibody at 1 ug/mL.

Immunohistochemistry-Paraffin: Siglec-11 Antibody [NBP1-77025] - Human liver tissue with Siglec11 antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Siglec-11 Antibody - BSA Free [NBP1-77025] - Immunofluorescence of SIGLEC11 in human liver tissue with SIGLEC11 antibody at 20 u/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Siglec-11 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Siglec-11 Antibody - BSA Free (NBP1-77025) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human Siglec11. The immunogen is located within the last 50 amino acids of Siglec11. Amino Acid Squence: WEPADQEAPSTTEYSEIK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIGLEC11
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: Despite its predicted size, Siglec-11 migrates at a higher than expected molecular weight in SDS-PAGE.
• Control Peptide:

  Siglec-11 Peptide (NBP1-77025PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Siglec-11 Antibody - BSA Free

• sialic acid binding Ig-like lectin 11
• sialic acid-binding Ig-like lectin 11
• Sialic acid-binding lectin 11
• Siglec11
• Siglec-11

Background

Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to alpha2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist. Siglec11 antibody will not cross-react with Siglec10. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIGLEC11
• Uniprot:
  • NP_443116()