Siglec-1/CD169 Antibody (645608) [Unconjugated]

Western blot shows lysates of mouse splenocytes. PVDF Membrane was probed with 1 µg/mL of Mouse Siglec-1/CD169 Monoclonal Antibody (Catalog # MAB5610) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Siglec-1/CD169 at approximately 180 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

RAW264 cells stimulated with 1 ug/mL LPS overnight were stained with Rat Anti-Mouse Siglec‑1/CD169 Monoclonal Antibody (Catalog # MAB5610, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clone: 645608
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Siglec-1/CD169 Antibody (645608) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Siglec-1/CD169
  Thr20-Leu1639 (predicted)
  Accession # Q62230
• Specificity: Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec-1,
  -5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SIGLEC1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-1/CD169 Antibody (645608) [Unconjugated]

• CD169
• FLJ00051
• sialic acid binding Ig-like lectin 1, sialoadhesin
• sialoadhesin
• Siglec1
• Siglec-1

Background

Siglec-1, also known as sialoadhesin or CD169, is a 175‑185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-1/CD169 Antibody (MAB5610)(6)

Publications using MAB5610

• Junqing Hu, Jinhao Xu, Mingyue Li, Yanping Zhang, Huaiqiang Yi, Jiangning Chen et al. Targeting Lymph Node Sinus Macrophages to Inhibit Lymph Node Metastasis Molecular Therapy - Nucleic Acids 2019-06-07 [PMID: 31121477] (Western Blot, Mouse)
• Y Wu, C Lan, D Ren, GY Chen Induction of Siglec-1 by Endotoxin Tolerance Suppresses the Innate Immune Response by Promoting TGF-?1 Production J. Biol. Chem., 2016-04-18;291(23):12370-82. 2016-04-18 [PMID: 27129263] (Flow Cytometry, Mouse)
• Zheng Q, Hou J, Zhou Y, Yang Y, Cao X Type I IFN-Inducible Downregulation of MicroRNA-27a Feedback Inhibits Antiviral Innate Response by Upregulating Siglec1/TRIM27. J Immunol, 2015-12-23;196(3):1317-26. 2015-12-23 [PMID: 26700765] (Western Blot, Mouse)
• Immunoactivation induced by chronic viral infection inhibits viral replication and drives immunosuppression through sustained IFN-I responses Eur. J. Immunol., 2015-11-17;46(2):372-80. 2015-11-17 [PMID: 26507703] (Flow Cytometry, IHC-Fr, Mouse)
• Zheng Q, Hou J, Zhou Y, Yang Y, Xie B, Cao X Siglec1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27. Cell Res, 2015-09-11;25(10):1121-36. 9/11/2015 [PMID: 26358190]
• Gerlini A, Colomba L, Furi L, Braccini T, Manso A, Pammolli A, Wang B, Vivi A, Tassini M, Van Rooijen N, Pozzi G, Ricci S, Andrew P, Koedel U, Moxon E, Oggioni M The role of host and microbial factors in the pathogenesis of pneumococcal bacteraemia arising from a single bacterial cell bottleneck. PLoS Pathog, 2014-03-20;10(3):e1004026. 2014-03-20 [PMID: 24651834] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: SIGLEC1
• Uniprot:
  • Q62230()