Siglec-1/CD169 Antibody (645608) [Phycoerythrin]

• Applications: Flow
• Clone: 645608
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

Siglec-1/CD169 Antibody (645608) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Siglec-1/CD169
  Thr20-Leu1639 (predicted)
  Accession # Q62230
• Specificity: Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec‑1/CD169, -5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SIGLEC1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Siglec-1, also known as Sialoadhesin or CD169, is a 175-185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIGLEC1