SIAE Antibody - BSA Free

Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Staining of human colon shows strong cytoplasmic positivity in lymphoid cells.

Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Staining of human kidney shows strong cytoplasmic granular positivity in cells in tubules.

Analysis using Anti-SIAE antibody HPA038053 (A) shows similar pattern to independent antibody HPA038052 (B).

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SIAE Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SIAE Antibody - BSA Free (NBP1-84371) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ACGVPKQGSIPYDSVTGPSKHSVLWNAMIHPLCNMTLKGVVWYQGESNINYNTDLYNCTFPALIEDWRETFHRGSQGQTERFFPFGLVQLSSDLS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIAE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  SIAE Recombinant Protein Antigen (NBP1-84371PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SIAE Antibody - BSA Free

• AIS6
• CSEC
• CSE-C
• cytosolic sialic acid 9-O-acetylesterase homolog
• EC 3.1.1.53
• H-Lse
• LSE
• MGC87009
• sialate O-acetylesterase
• sialic acid acetylesterase
• Sialic acid-specific 9-O-acetylesterase
• sialic acid-specific acetylesterase II
• Ysg2 homolog (mouse)
• YSG2

Background

Sialic acids are acidic 9-carbon sugars typically found at the nonreducing end of sugar chains. They are frequently modified by 9-O-acetylation, and this modification is removed by sialic acid acetylesterases. SIAE appears to encode both lysosomal and cytosolic sialic acid acetylesterase isoforms (LSE and CSE, respectively) (Takematsu et al., 1999 [PubMed 10464298]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIAE