SHPRH Antibody (OTI5G8) - Azide and BSA Free

Western Blot: SHPRH Antibody (OTI5G8) - Azide and BSA Free [NBP2-74180] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Flow Cytometry: SHPRH Antibody (OTI5G8) - Azide and BSA Free [NBP2-74180] - Analysis of Jurkat cells, using anti-SHPRH antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, CyTOF-ready
• Clone: OTI5G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SHPRH Antibody (OTI5G8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SHPRH Antibody (OTI5G8) - Azide and BSA Free (NBP2-02797) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-100 and 1500-1600 of human SHPRH (NP_775105) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SHPRH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Western Blot 1:2000
• Theoretical MW: 192.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SHPRH Antibody (OTI5G8) - Azide and BSA Free

• 2610103K11Rik
• bA545I5.2
• E3 ubiquitin-protein ligase SHPRH
• EC 3.6.4.-
• EC 6.3.2.-
• FLJ27258
• FLJ37625
• FLJ90837
• histone-linker, PHD and RING finger domain-containing helicase
• KIAA2023FLJ45012
• MGC134886
• SNF2 histone linker PHD RING helicase

Background

SHPRH is a novel gene containing SNF2, PHD-finger, RING-finger, helicase, and linker histone domains termed SHPRH, is a possible candidate for a tumor suppressor gene. These domains are characteristic of several DNA repair proteins, transcription factors, and helicases. Mapping of this gene to 6q24, a region reported to contain a tumor suppressor locus suggests a role in tumor suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHPRH