SHIP [p Tyr1020] Antibody (SR1167) - BSA Free

Western Blot: SHIP [p Tyr 1020] Antibody (SR1167) [NBP3-21565] - Western blot analysis using NBP3-21565 in (1) Raji cell lysate; (2) Raji cell treated with pervanadate lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clone: SR1167
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SHIP [p Tyr1020] Antibody (SR1167) - BSA Free Summary

• Description: Novus Biologicals Rabbit SHIP [p Tyr1020] Antibody (SR1167) - BSA Free (NBP3-21565) is a recombinant monoclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-SHIP [Tyr1020] (Uniprot #: Q92835)
• Modification: p Tyr1020
• Specificity: Detects endogenous levels of phospho-SHIP [Tyr1020]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: INPP5D
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:20
  • Western Blot 1:1000-1:2000
• Theoretical MW: 145 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SHIP [p Tyr1020] Antibody (SR1167) - BSA Free

• 145kD
• EC 3.1.3
• hp51CNMGC142142
• Inositol polyphosphate-5-phosphatase of 145 kDa
• inositol polyphosphate-5-phosphatase, 145kDa
• INPP5D
• MGC104855
• SHIP
• SHIPMGC142140

Background

SH2-containing inositol phosphatase 1 (SHIP) is a 145 kDa hemopoietic-specific phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3, 4-bisphosphate. SHIP has been shown to bind directly a phosphorylated motif, immunoreceptor tyrosine-based inhibition motif (ITIM), present in the cytoplasmic domain of FcgammaRIIB1 (1). Also, results demonstrate that FcgammaRIIB use SHIP1 to inhibit pathways shared by receptor tyrosine kinases and immunoreceptors to trigger cell proliferation and cell activation, respectively (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INPP5D