SHIP Antibody (9G11) - BSA Free

Immunoprecipitation: SHIP Antibody (9G11) [NBP3-26196] - Immunoprecipitating SHIP in Raji whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26196 in Raji whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26196 (2ug) + Raji whole cell lysate(500ug). Lane 3: Raji whole cell lysate (10ug).

Immunohistochemistry: SHIP Antibody (9G11) [NBP3-26196] - Image of SHIP Antibody (9G11) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: SHIP Antibody (9G11) [NBP3-26196] - Positive Western Blot detected in: Raji whole cell lysate, K562 whole cell lysate. All lanes: SHIP Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 134, 110 kDa Observed band size: 145 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 9G11
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SHIP Antibody (9G11) - BSA Free Summary

• Description: Novus Biologicals Rabbit SHIP Antibody (9G11) - BSA Free (NBP3-26196) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human SHIP [UniProt Q92835]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: INPP5D
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SHIP Antibody (9G11) - BSA Free

• 145kD
• EC 3.1.3
• hp51CNMGC142142
• Inositol polyphosphate-5-phosphatase of 145 kDa
• inositol polyphosphate-5-phosphatase, 145kDa
• INPP5D
• MGC104855
• SHIP
• SHIPMGC142140

Background

SH2-containing inositol phosphatase 1 (SHIP) is a 145 kDa hemopoietic-specific phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3, 4-bisphosphate. SHIP has been shown to bind directly a phosphorylated motif, immunoreceptor tyrosine-based inhibition motif (ITIM), present in the cytoplasmic domain of FcgammaRIIB1 (1). Also, results demonstrate that FcgammaRIIB use SHIP1 to inhibit pathways shared by receptor tyrosine kinases and immunoreceptors to trigger cell proliferation and cell activation, respectively (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INPP5D