SHC1 Antibody (OTI3A1) - Azide and BSA Free

Western Blot: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHC1.

Immunocytochemistry/Immunofluorescence: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1 .

Immunohistochemistry: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Staining of paraffin-embedded lung tissue using anti-SHC1 mouse monoclonal antibody.

Western Blot: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Figure from citation:(p66shc) protein level by using anti-SHC1 antibody in human HBL-100/MCF-7 cell lysates.

Immunohistochemistry: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Staining of paraffin-embedded bladder tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Staining of paraffin-embedded breast tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Staining of paraffin-embedded colon tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Staining of paraffin-embedded Kidney tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry: SHC1 Antibody (OTI3A1) - Azide and BSA Free [NBP2-74167] - Staining of paraffin-embedded prostate tissue using anti-SHC1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: OTI3A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SHC1 Antibody (OTI3A1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SHC1 Antibody (OTI3A1) - Azide and BSA Free (NBP2-02434) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SHC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:2000
• Theoretical MW: 51.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SHC1 Antibody (OTI3A1) - Azide and BSA Free

• FLJ26504
• p66
• p66SHC
• SH2 domain protein C1
• SHC (Src homology 2 domain containing) transforming protein 1
• SHC (Src homology 2 domain-containing) transforming protein 1
• SHC
• SHC1
• SHCA
• SHC-transforming protein 1
• SHC-transforming protein 3
• SHC-transforming protein A
• Src homology 2 domain-containing-transforming protein C1

Background

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHC1