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SHANK3 Antibody (S69-46) [FITC]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clone
S69-46
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-47610AF405
Alexa Fluor 488
NBP1-47610AF488
Alexa Fluor 647
NBP1-47610AF647
Alexa Fluor 700
NBP1-47610AF700
Biotin
NBP1-47610B
DyLight 350
NBP1-47610UV
DyLight 405
NBP1-47610V
DyLight 488
NBP1-47610G
DyLight 550
NBP1-47610R
DyLight 650
NBP1-47610C
DyLight 680
NBP1-47610FR
DyLight 755
NBP1-47610IR
HRP
NBP1-47610H
Janelia Fluor 549
NBP1-47610JF549
Janelia Fluor 646
NBP1-47610JF646
Unconjugated
NBP1-47610
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SHANK3 Antibody (S69-46) [FITC] Summary

Immunogen
Synthetic peptide amino acids 840-857 of rat Shank3
Localization
Cytoplasm, Cell Junction, Synapse, Postsynaptic Cell Membrane, Postsynaptic Density
Specificity
Detects approx 190kDa. No cross-reactivity against Shank1 or Shank2.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SHANK3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for SHANK3 Antibody (S69-46) [FITC]

  • DEL22q13.3
  • KIAA1650proline rich synapse associated protein 2
  • Proline-rich synapse-associated protein 2
  • prosap2
  • PSAP2SH3 and multiple ankyrin repeat domains protein 3
  • SH3 and multiple ankyrin repeat domains 3
  • shank postsynaptic density protein
  • Shank3
  • SPANK-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: SHANK1 Antibody [NB300-167] - Synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) and VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar =300 um. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY licence.
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Mouse retina using NB300-167.
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(6 Publications)
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Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures. H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) and (I) showing postsynaptic labeling of GluA1. Scale bar (valid for all images): 10 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140868), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: PSD-95 Antibody (6G6-1C9) [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 Double labeling with antibodies against syntaxin-1 (D) and synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY licence.
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NBP1-76911
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Immunocytochemistry/Immunofluorescence: GKAP/DLGAP1 Antibody [NBP1-76911] - Human Brain cells
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Src [p Tyr419]
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Neuroligin 3/NLGN3
Antibody
NBP1-90080
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I/CA1 Antibody
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SHANK3 Antibody
(S69-46) [FITC]
NBP1-47610F
SHANK3 Antibody (S69-46) [FITC]
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P

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Product General Protocols

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ICC/IF Video Protocol

FAQs for SHANK3 Antibody (NBP1-47610F) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP1-47610AF405
Alexa Fluor 488 NBP1-47610AF488
Alexa Fluor 647 NBP1-47610AF647
Alexa Fluor 700 NBP1-47610AF700
Biotin NBP1-47610B
DyLight 350 NBP1-47610UV
DyLight 405 NBP1-47610V
DyLight 488 NBP1-47610G
DyLight 550 NBP1-47610R
DyLight 650 NBP1-47610C
DyLight 680 NBP1-47610FR
DyLight 755 NBP1-47610IR
FITC NBP1-47610F
HRP NBP1-47610H
Janelia Fluor 549 NBP1-47610JF549
Janelia Fluor 646 NBP1-47610JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol SHANK3
Entrez
Uniprot