SH3BP5 Antibody - BSA Free

Western Blot: SH3BP5 Antibody [NBP2-20343] - Sample (30 ug of whole cell lysate) A: THP-1 12% SDS PAGE gel, diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SH3BP5(NBP2-20343) antibody at 1:250 dilution. br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SH3BP5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SH3BP5 Antibody - BSA Free (NBP2-20343) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human SH3BP5 Recombinant protein.
• Localization: Mitochondrion
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SH3BP5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SH3BP5 Antibody - BSA Free

• Sab
• SABSH3BP-5
• SH3 binding protein
• SH3 domain-binding protein 5
• SH3 domain-binding protein that preferentially associates with BTK
• SH3-domain binding protein 5 (BTK-associated)

Background

SH3BP5 (SH3 domain binding protein 5) binds to the SH3 domain of Bruton tyrosine kinase (BTK), a cytoplasmic tyrosine kinase crucial for the maturation of B-lineage cells. It inhibits the auto- and transphosphorylation activity of BTK and negatively regulates BTK-related cytoplasmic signaling in B-cells. SH3BP5 also interacts with JNK. The interaction with JNK occurs through a mitogen-activated protein KIM (kinase interaction motif) in a region distinct from the Bruton's tyrosine kinase-binding domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SH3BP5
• Uniprot:
  • O60239(Human)