SFRS9 Antibody

Western Blot: SFRS9 Antibody [NBP2-13385] - Analysis in human cell line NB4.

Immunocytochemistry/Immunofluorescence: SFRS9 Antibody [NBP2-13385] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SFRS9 Antibody [NBP2-13385] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: SFRS9 Antibody [NBP2-13385] - Analysis in human testis and skeletal muscle tissues using NBP2-13385 antibody. Corresponding SRSF9 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: SFRS9 Antibody [NBP2-13385] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: SFRS9 Antibody [NBP2-13385] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: SFRS9 Antibody [NBP2-13385] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Product Discontinued

SFRS9 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PRTYGGRGGWPRGGRNGPPTRRSDF
• Predicted Species: Mouse (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRSF9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunofluorescence, Fixation/Permeabilization: PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SFRS9 Antibody

• Pre-mRNA-splicing factor SRp30C
• serine/arginine-rich splicing factor 9
• SFRS9
• Splicing factor, arginine/serine-rich 9SR splicing factor 9
• SRp30c

Background

SFRS9 plays a role in constitutive splicing and can modulate the selection of alternative splice sites

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRSF9
• Entrez:
  • 8683(Human)
• Uniprot:
  • Q13242(Human)